Evidence map›Paper›PMID 36609619›Full record

ArticleScientific reports2023

Unique insertion/deletion polymorphisms within histidine-rich region of histidine-rich glycoprotein in Thoroughbred horses.

Ryo Muko, Tomoya Sunouchi, Shuntaro Urayama, Yuko Toishi, Kanichi Kusano, Hiroaki Sato, Masanori Muranaka, Taekyun Shin, Masa-Aki Oikawa, Yoshinobu Ojima and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 3 countries.

Ryo MukoInstitute of Global Innovation Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Tomoya SunouchiLaboratory of Comparative Animal Medicine, Division of Animal Life Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan.
Shuntaro UrayamaRace Horse Clinic, Ritto Training Center, Japan Racing Association, Shiga, Japan.
Yuko ToishiShadai Stallion Station, Shadai Corporation, Hokkaido, Japan.
Kanichi KusanoRace Horse Clinic, Ritto Training Center, Japan Racing Association, Shiga, Japan.
Hiroaki SatoRace Integrity Section, Stewards Department, Japan Racing Association, Tokyo, Japan.
Masanori MuranakaRace Horse Clinic, Ritto Training Center, Japan Racing Association, Shiga, Japan.
Taekyun ShinDepartment of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju, South Korea.
Masa-Aki OikawaDiagnostic Laboratory, Equine Veterinary Medical Center, Education City, Doha, Qatar.
Yoshinobu OjimaInstitute of Global Innovation Research, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Mohammad AliDiagnostic Laboratory, Equine Veterinary Medical Center, Education City, Doha, Qatar.
Yoshihiro NomuraScleroprotein and Leather Research Institute, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Hiroshi MatsudaLaboratory of Comparative Animal Medicine, Division of Animal Life Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo, 183-8509, Japan.
Akane TanakaInstitute of Global Innovation Research, Tokyo University of Agriculture and Technology, Tokyo, Japan. akane@cc.tuat.ac.jp.
Tokyo University of Agriculture and Technology · JPJapan Racing Association · JPEquine Veterinary Medical Center · QAJeju National University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Histidine-rich glycoprotein (HRG) is abundant plasma protein with various effects on angiogenesis, coagulation, and immune responses. Previously, we identified the base and amino acid sequences of equine HRG (eHRG) and revealed that eHRG regulates neutrophil functions. In this study, we first conducted a large-scale gene analysis with DNA samples extracted from 1700 Thoroughbred horses and identified unique insertion/deletion polymorphisms in the histidine-rich region (HRR) of eHRG. Here we report two types of polymorphisms (deletion type 1 [D1] and deletion type 2 [D2]) containing either a 45 bp or 90 bp deletion in the HRR of eHRG, and five genotypes of eHRG (insertion/insertion [II], ID1, ID2, D1D1, and D1D2) in Thoroughbred horses. Allele frequency of I, D1, and D2, was 0.483, 0.480, and 0.037 and the incidence of each genotype was II: 23.4%, ID1: 46.2%, ID2: 3.6%, D1D1: 23.1%, and D1D2: 3.7%, respectively. The molecular weights of each plasma eHRG protein collected from horses with each genotype was detected as bands of different molecular size, which corresponded to the estimated amino acid sequence. The nickel-binding affinity of the D1 or D2 deletion eHRG was reduced, indicating a loss of function at the site. eHRG proteins show a variety of biological and immunological activities in vivo, and HRR is its active center, suggesting that genetic polymorphisms in eHRG may be involved in the performance in athletic ability, productivity, and susceptibility to infectious diseases in Thoroughbred horses.

Indexed as

Blood ProteinsHistidineAmino Acid SequenceAnimalsHistidine-Rich GlycoproteinHorsesPolymorphism, GeneticBlood ProteinsHistidineHistidine-Rich Glycoprotein

Identifiers

PMID36609619
PMCPMC9822902
OpenAlexW4313595717

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.