Evidence map›Paper›PMID 36609316›Full record

ArticleBiotechnology for biofuels and bioproducts2023

Towards the rate limit of heterologous biotechnological reactions in recombinant cyanobacteria.

Giovanni Davide Barone, Michal Hubáček, Lenny Malihan-Yap, Hanna C Grimm, Lauri Nikkanen, Catarina C Pacheco, Paula Tamagnini, Yagut Allahverdiyeva, Robert Kourist

Open access · goldAbstract read
In one paragraph

Article in Biotechnology for biofuels and bioproducts, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 15 citations in OpenAlex.

  1. Toward Sustainability: Intensification of Light-Driven Whole Cell Biocatalysis.Chembiochem : a European journal of chemical biology · 2026
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  4. Optimising COMicrobial cell factories · 2025
    Article
  5. Article
  6. Article
  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 3 countries.

Giovanni Davide BaroneBiocatalysis and Protein Engineering, Institute of Molecular Biotechnology, Graz University of Technology, 8010, Graz, Austria.
Michal HubáčekLaboratory of Molecular Plant Biology, Department of Life Technologies, University of Turku, 20014, Turku, Finland.
Lenny Malihan-YapBiocatalysis and Protein Engineering, Institute of Molecular Biotechnology, Graz University of Technology, 8010, Graz, Austria.
Hanna C GrimmBiocatalysis and Protein Engineering, Institute of Molecular Biotechnology, Graz University of Technology, 8010, Graz, Austria.
Lauri NikkanenLaboratory of Molecular Plant Biology, Department of Life Technologies, University of Turku, 20014, Turku, Finland.
Catarina C Pachecoi3S - Instituto de Investigação e Inovação em Saúde, IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135, Porto, Portugal.
Paula Tamagninii3S - Instituto de Investigação e Inovação em Saúde, IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, 4200-135, Porto, Portugal.
Yagut AllahverdiyevaLaboratory of Molecular Plant Biology, Department of Life Technologies, University of Turku, 20014, Turku, Finland.
Robert KouristBiocatalysis and Protein Engineering, Institute of Molecular Biotechnology, Graz University of Technology, 8010, Graz, Austria. kourist@tugraz.at.
University of Turku · FIGraz University of Technology · ATi3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto · PTInstitute of Molecular Biotechnology · ATUniversidade do Porto · PT

Funding

H2020 Marie Skłodowska-Curie Actions 764920Horizon 2020 Framework Programme 899576
6 · The paper itself

Abstract

backgroundCyanobacteria have emerged as highly efficient organisms for the production of chemicals and biofuels. Yet, the productivity of the cell has been low for commercial application. Cyanobacterial photobiotransformations utilize photosynthetic electrons to form reducing equivalents, such as NADPH-to-fuel biocatalytic reactions. These photobiotransformations are a measure to which extent photosynthetic electrons can be deviated toward heterologous biotechnological processes, such as the production of biofuels. By expressing oxidoreductases, such as YqjM from Bacillus subtilis in Synechocystis sp. PCC 6803, a high specific activity was obtained in the reduction of maleimides. Here, we investigated the possibility to accelerate the NAD(P)H-consuming redox reactions by addition of carbohydrates as exogenous carbon sources such as D-Glucose under light and darkness.

resultsA 1.7-fold increase of activity (150 µmol min

conclusionsResults show that under photoautotrophic conditions at a specific activity of 90 µmol min

Indexed as

BiotransformationsCyanobacteriaD-GlucoseEne-reductionSynechocystis sp. PCC 6803

Identifiers

PMID36609316
PMCPMC9825001
OpenAlexW4313650921

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.