Evidence map›Paper›PMID 36609254›Full record

ArticleBMC cancer2023

Analysis of the expression and prognostic significance of DDK complex in Hepatocarcinoma.

Min Wang, Zu-Hua Qiu, Yu-Zhuo Wang, Bo Lian, Jing-Kun Bai, Yong-Jie Zhou, Hong-Jie Ji

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 100% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 0 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Min Wang *School of Life Science and Technology, Weifang Medical University, No.7166, Baotongxi Street, Weifang, 261053, China.
Zu-Hua Qiu *School of Life Science and Technology, Weifang Medical University, No.7166, Baotongxi Street, Weifang, 261053, China.
Yu-Zhuo WangSchool of Life Science and Technology, Weifang Medical University, No.7166, Baotongxi Street, Weifang, 261053, China.
Bo LianSchool of Life Science and Technology, Weifang Medical University, No.7166, Baotongxi Street, Weifang, 261053, China.
Jing-Kun BaiSchool of Life Science and Technology, Weifang Medical University, No.7166, Baotongxi Street, Weifang, 261053, China.
Yong-Jie ZhouLaboratory of Liver Transplantation, West China Hospital, Sichuan University, No.1, Keyuan 4 Road, Chengdu, 610041, China. yongjiezhou@scu.edu.cn.
Hong-Jie JiSchool of Life Science and Technology, Weifang Medical University, No.7166, Baotongxi Street, Weifang, 261053, China. jihongjie@wfmc.edu.cn.
Weifang Medical University · CNWest China Hospital of Sichuan University · CN

Funding

National Natural Science Foundation of China 82002600Weifang Municipal Science and Technology Development Plan Project 2020GX018Weifang Municipal Science and Technology Development Plan Project 2020YX045Youth Project of Natural Science Foundation of Shandong Province ZR2020QC094
6 · The paper itself

Abstract

backgroundHepatocellular carcinoma (HCC) remains one of the most common and lethal malignancies worldwide. Although DBF4-dependent kinase (DDK) complex composed of CDC7 kinase and its regulatory subunit DBF4 has been shown to be overexpressed in primary tumors and promotes tumor development, while its role and prognostic value in HCC remain largely unknown. In the present study, the expression of DBF4 and CDC7 and their relationship with clinical characteristics were comprehensively analyzed.

methodsThe mRNA expression profiles of HCC and the corresponding clinical data of HCC patients were downloaded from TCGA and GEO databases, respectively. The differences in DBF4 and CDC7 expression in tumor tissues and adjacent normal tissues were analyzed. HCC-derived tissue microarray (TMA) was used to evaluate and score the expression of CDC7 by immunohistochemistry (IHC) staining. The Kaplan-Meier method and the Cox regression method were used to analyze the relationship between overall survival and clinical characteristics of the patients. Gene set enrichment analysis (GSEA) was used to analyze the pathway enrichment of DBF4 and CDC7.

resultsDBF4 and CDC7 had similar expression patterns in HCC patients. Detailly, compared with adjacent tissues, both mRNA and protein of DBF4 and CDC7 were significantly higher in HCC, and their expression was positively correlated with AJCC_T stage, clinical stage and G stage (grade) of liver cancer patients, and higher DBF4 or CDC7 expression predicted a worse prognosis in HCC patients with shorter overall survival (OS), recurrence-free survival (RFS), disease-specific survival (DSS) and progress-free survival (PFS). Cox regression analysis suggested that both DBF4 and CDC7 were independent risk factors for the prognosis of HCC patients in TCGA dataset. GSEA suggested that both DBF4 and CDC7 were positively correlated with cell cycle and DNA replication. Finally, the prognostic value of CDC7 was furtherly confirmed by TMA-based IHC staining results.

conclusionsOur study showed that DDK complex was significantly increased in HCC. Both DBF4 and CDC7 may be potential diagnostic and prognostic markers for HCC, and high expression of DDK members predicts a worse prognosis in patients with HCC, which may be associated with high tumor cell proliferation rate.

Indexed as

Carcinoma, HepatocellularLiver NeoplasmsCell Cycle ProteinsHumansPrognosisProtein Serine-Threonine KinasesCDC7 protein, humanCell Cycle ProteinsProtein Serine-Threonine KinasesCDC7DBF4DDKDNA replicationHepatocellular carcinoma

Identifiers

PMID36609254
PMCPMC9817372
OpenAlexW4313640806

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.