ArticleMolecular plant2023
A chromosome-level genome assembly reveals that a bipartite gene cluster formed via an inverted duplication controls monoterpenoid biosynthesis in Schizonepeta tenuifolia.
Article in Molecular plant, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 33 citations in OpenAlex.
- Expansion and functional diversification of terpene synthases shape volatile terpenoid landscape inActa pharmaceutica Sinica. B · 2026Article
- MYB Transcription Factors Negatively RegulatePlants (Basel, Switzerland) · 2026Article
- Research on the epigenetic alterations inFrontiers in plant science · 2026Article
- Synteny Analysis and Visualization of Gene Clusters.Methods in molecular biology (Clifton, N.J.) · 2026Article
- NPlant biotechnology journal · 2025Article
- Phylogenomics and metabolic engineering reveal a conserved gene cluster in Solanaceae plants for withanolide biosynthesis.Nature communications · 2025Article
- From data to discovery: leveraging big data in plant natural products biosynthesis research.The Plant journal : for cell and molecular biology · 2025Review
- Haplotype-resolved genome ofHorticulture research · 2025Article
- Advances in synthesizing plant-derived isoflavones and their precursors with multiple pharmacological activities using engineered yeasts.Microbial cell factories · 2025Review
- Subfunctionalization and epigenetic regulation of a biosynthetic gene cluster inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Insights into the Genomic Background of Nine Common Chinese Medicinal Plants by Flow Cytometry and Genome Survey.Plants (Basel, Switzerland) · 2024Article
- Genome mining of metabolic gene clusters in the Rubiaceae family.Computational and structural biotechnology journal · 2024Article
- Pseudomolecule-scale genome assemblies of Drepanocaryum sewerzowii and Marmoritis complanata.G3 (Bethesda, Md.) · 2024Article
- Impact of Methyl Jasmonate on Terpenoid Biosynthesis and Functional Analysis of Sesquiterpene Synthesis Genes inPlants (Basel, Switzerland) · 2024Article
- A haplotype-resolved gap-free genome assembly provides novel insight into monoterpenoid diversification inHorticulture research · 2024Article
- Unraveling the specialized metabolic pathways in medicinal plant genomes: a review.Frontiers in plant science · 2024Review
- Dynamic evolution of terpenoid biosynthesis in the Lamiaceae.Molecular plant · 2023Article
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12 authors at 3 institutions in 3 countries.
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Abstract
Biosynthetic gene clusters (BGCs) are regions of a genome where genes involved in a biosynthetic pathway are in proximity. The origin and evolution of plant BGCs as well as their role in specialized metabolism remain largely unclear. In this study, we have assembled a chromosome-scale genome of Japanese catnip (Schizonepeta tenuifolia) and discovered a BGC that contains multiple copies of genes involved in four adjacent steps in the biosynthesis of p-menthane monoterpenoids. This BGC has an unprecedented bipartite structure, with mirrored biosynthetic regions separated by 260 kilobases. This bipartite BGC includes identical copies of a gene encoding an old yellow enzyme, a type of flavin-dependent reductase. In vitro assays and virus-induced gene silencing revealed that this gene encodes the missing isopiperitenone reductase. This enzyme evolved from a completely different enzyme family to isopiperitenone reductase from closely related Mentha spp., indicating convergent evolution of this pathway step. Phylogenomic analysis revealed that this bipartite BGC has emerged uniquely in the S. tenuifolia lineage and through insertion of pathway genes into a region rich in monoterpene synthases. The cluster gained its bipartite structure via an inverted duplication. The discovered bipartite BGC for p-menthane biosynthesis in S. tenuifolia has similarities to the recently described duplicated p-menthane biosynthesis gene pairs in the Mentha longifolia genome, providing an example of the convergent evolution of gene order. This work expands our understanding of plant BGCs with respect to both form and evolution, and highlights the power of BGCs for gene discovery in plant biosynthetic pathways.
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