Evidence map›Paper›PMID 36607795›Full record

ArticleAmerican journal of physiology. Heart and circulatory physiology2023

17β-Estradiol promotes sex-specific dysfunction in isolated human arterioles.

Gopika SenthilKumar, Boran Katunaric, Henry Bordas-Murphy, Micaela Young, Erin L Doren, Mary E Schulz, Michael E Widlansky, Julie K Freed

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Heart and circulatory physiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
4.0field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 20 citations in OpenAlex.

  1. Guidelines for evaluating endothelial function in vascular tissue.American journal of physiology. Heart and circulatory physiology · 2026
    Review
  2. Article
  3. Article
  4. Review
  5. Review
  6. Overexpression of adipose tissue ERα enhances PVAT anticontractility via NOX4-derived HAmerican journal of physiology. Heart and circulatory physiology · 2025
    Article
  7. Promoting Resiliency to Stress in the Vascular Endothelium.Basic & clinical pharmacology & toxicology · 2025
    Review
  8. Article
  9. Article
  10. Review
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Gopika SenthilKumarDepartment of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin, United States.ORCID 0000-0001-5781-1056
Boran KatunaricCardiovascular Center, Medical College of Wisconsin, Milwaukee, Wisconsin, United States.ORCID 0000-0002-4302-6409
Henry Bordas-MurphyCardiovascular Center, Medical College of Wisconsin, Milwaukee, Wisconsin, United States.
Micaela YoungDepartment of Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin, United States.
Erin L DorenDepartment of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin, United States.
Mary E SchulzCardiovascular Center, Medical College of Wisconsin, Milwaukee, Wisconsin, United States.
Michael E WidlanskyCardiovascular Center, Medical College of Wisconsin, Milwaukee, Wisconsin, United States.ORCID 0000-0002-1461-9707
Julie K FreedDepartment of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin, United States.ORCID 0000-0002-9367-2281
Medical College of Wisconsin · US

Funding

NOVEL ROLE OF SPHINGOLIPIDS IN MAINTAINING VASCULAR HOMEOSTASISK08HL141562 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI FREED, JULIE K · 2019 to 2023
$851k
NHLBI NIH HHS K08 HL141562
6 · The paper itself

Abstract

Despite data showing that estrogen is vasculoprotective in large conduit arteries, hormone therapy (HT) during menopause has not proven to mitigate cardiovascular disease (CVD) risk. Estrogen exposure through prolonged oral contraceptive use and gender-affirming therapy can also increase cis- and trans-females' risk for future CVD, respectively. The microvasculature is a unique vascular bed that when dysfunctional can independently predict future adverse cardiac events; however, studies on the influence of estrogen on human microvessels are limited. Here, we show that isolated human arterioles from females across the life span maintain nitric oxide (NO)-mediated dilation to flow, whereas chronic (16-20 h) exposure to exogenous (100 nM) 17β-estradiol promotes microvascular endothelial dysfunction in vessels from adult females of <40 and ≥40 yr of age. The damaging effect of estrogen was more dramatic in arterioles from biological males, as they exhibited both endothelial and smooth muscle dysfunction. Furthermore, females of <40 yr have greater endothelial expression of estrogen receptor-β (ER-β) and G protein-coupled estrogen receptor (GPER) compared with females of ≥40 yr and males. Estrogen receptor-α (ER-α), the prominent receptor associated with protective effects of estrogen, was identified within the adventitia as opposed to the endothelium across all groups. To our knowledge, this is the first study to report the detrimental effects of estrogen on the human microvasculature and highlights differences in estrogen receptor expression.

Indexed as

Receptors, EstrogenVascular DiseasesAdultArteriolesEndothelium, VascularEstradiolEstrogen Receptor alphaEstrogensFemaleHumansMaleReceptors, G-Protein-CoupledVasodilationEstradiolEstrogen Receptor alphaEstrogensReceptors, EstrogenReceptors, G-Protein-Coupledendotheliumestrogenflow-induced dilationhormone therapymicrocirculation

Identifiers

PMID36607795
PMCPMC9925162
OpenAlexW4313597960

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.