ArticleThe Journal of comparative neurology2023
Monosynaptic restriction of the anterograde herpes simplex virus strain H129 for neural circuit tracing.
Article in The Journal of comparative neurology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 12 citations in OpenAlex.
- Article
- PVNAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Transsynaptic viral tools in neural circuit analysis: from anatomical mapping to functional interrogation.Frontiers in neural circuits · 2026Review
- Updated Toolbox for Assessing Neuronal Network Reconstruction after Cell Therapy.Bioengineering (Basel, Switzerland) · 2024Review
- Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing.eLife · 2024Article
- Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA andbioRxiv : the preprint server for biology · 2023Article
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
Identification of synaptic partners is a fundamental task for systems neuroscience. To date, few reliable techniques exist for whole brain labeling of downstream synaptic partners in a cell-type-dependent and monosynaptic manner. Herein, we describe a novel monosynaptic anterograde tracing system based on the deletion of the gene UL6 from the genome of a cre-dependent version of the anterograde Herpes Simplex Virus 1 strain H129. Given that this knockout blocks viral genome packaging and thus viral spread, we reasoned that co-infection of a HSV H129 ΔUL6 virus with a recombinant adeno-associated virus expressing UL6 in a cre-dependent manner would result in monosynaptic spread from target cre-expressing neuronal populations. Application of this system to five nonreciprocal neural circuits resulted in labeling of neurons in expected projection areas. While some caveats may preclude certain applications, this system provides a reliable method to label postsynaptic partners in a brain-wide fashion.
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