ArticleMethods in molecular biology (Clifton, N.J.)2023
Using Budding Yeast as a Model to Understand Dynein-Mediated Cargo Transport.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- A nucleotide code governs Lis1's ability to relieve dynein autoinhibition.Nature chemical biology · 2026Article
- Conserved roles for the dynein intermediate chain and Ndel1 in assembly and activation of dynein.Nature communications · 2023Article
- Conserved Roles for the Dynein Intermediate Chain and Ndel1 in Assembly and Activation of Dynein.bioRxiv : the preprint server for biology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Cytoplasmic dynein-1 is a minus end-directed microtubule motor that transports numerous cargoes in cell types throughout the evolutionary spectrum. Dynein is regulated by various motor-intrinsic and motor-extrinsic factors that enhance its processivity, recruit it to various cellular sites, or otherwise promote or restrict its activity. Studying dynein activity in higher eukaryotes is complicated by various factors, including the myriad functions in which this motor participates, and the consequential pleotropic effects associated with disrupting its activity. Budding yeast has long been a powerful model system for understanding this enormous motor protein complex, which is highly conserved between yeast and humans at the primary sequence and structural levels. Studies in budding yeast are simplified by the fact that dynein only performs one known function in this organism: to position the mitotic spindle at the site of cell division. Monitoring dynein-mediated spindle movements in budding yeast provides a powerful tool for the quantitative measurements of various motility parameters, and a system with which to assess the consequence of mutations in dynein or its regulators. Here, we provide detailed protocols to perform quantitative measurements of dynein activity in live cells using a combination of fluorescence microscopy and computational methods to track and quantitate dynein-mediated spindle movements. These methods are broadly applicable to anyone that wishes to perform fluorescence microscopy on budding yeast.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.