Evidence map›Paper›PMID 36602331›Full record

ArticleMicrobiology spectrum2023

Identification and Characterization of Klebsiella pneumoniae from Farmed American Bullfrogs (

Han Lin, Jie Ma, Jingyang Sun, Zhendong Qin, Biao Jiang, Wei Li, Qing Wang, Youlu Su, Li Lin, Chun Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Virulence and resistance adaptation of Streptococcus agalactiae Ib/ST261 infecting bullfrogs in Hainan, China between 2019 and 2023.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026
    Article
  3. Article
  4. Field Prevalence and Pathological Features ofAnimals : an open access journal from MDPI · 2025
    Article
  5. Identification and characterization ofMicrobiology spectrum · 2025
    Article
  6. A Duplex PCR Assay for Rapid Detection ofFoodborne pathogens and disease · 2024
    Article
  7. Hypermucoviscous Multidrug-ResistantPathogens (Basel, Switzerland) · 2024
    Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Han LinGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Jie MaGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Jingyang SunGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Zhendong QinGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Biao JiangGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Wei LiGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Qing WangKey Laboratory of Aquatic Animal Immune Technology of Guangdong Province and Key Laboratory of Fishery Drug Development of Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, China.
Youlu SuGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Li LinGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
Chun LiuGuangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, Innovative Institute of Animal Healthy Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, China.ORCID 0000-0002-0070-7984
Zhongkai University of Agriculture and Engineering · CNChinese Academy of Fishery Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Klebsiella pneumoniae is a major cause of nosocomial infection and is considered a clinically important bacterium with antibiotic-resistant strains. There are few reports of K. pneumoniae infections in cultured aquatic animals, and no natural infection has been reported in amphibians. From September to October 2021, a high-mortality disease outbreak occurred in a pond-raised American bullfrog farm in Guangzhou, China. The infected bullfrogs were characterized by multiple organ congestive enlargement and inflammation. A pathogenic bacterium was isolated from the viscera of infected bullfrogs and confirmed to be K. pneumoniae by morphological, biochemical, and phylogenetic analyses. Infection experiments confirmed the virulence of the pathogenic strain against bullfrogs and tadpoles. A histopathological examination showed that the strain was harmful to multiple organs. Antibiotic resistance experiments indicated the isolate was a carbapenemase-producing multidrug-resistant K. pneumoniae (MDR-KP) strain. This study is the first report of K. pneumoniae infected American bullfrogs (Rana catesbeiana) and amphibians. These results will shed light on the pathogenicity of K. pneumoniae and help prevent and control K. pneumoniae infections in bullfrogs.

Indexed as

Klebsiella InfectionsKlebsiella pneumoniaeAnimalsAnti-Bacterial Agentsbeta-LactamasesFarmsHumansMicrobial Sensitivity TestsPhylogenyRana catesbeianaAnti-Bacterial Agentsbeta-LactamasesAmerican bullfrogKlebsiella pneumoniaemultidrug-resistantmultiple organ congestive enlargement

Identifiers

PMID36602331
PMCPMC9927386
OpenAlexW4313546139

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.