ArticlePlant biotechnology journal2023
Artificial nanovesicles for dsRNA delivery in spray-induced gene silencing for crop protection.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
63 citing papers in PubMed, 112 citations in OpenAlex.
- Cross-kingdom RNA in plant-microbe interactions: from molecular interactions to next-generation crop protection.Stress biology · 2026Review
- Advancing the adoption of RNA interference-based biopesticides.Nature plants · 2026Review
- Interactional Endocytosis and Transmembrane Transport Promote Cellular Internalization of Nano-Delivered RNA Drugs for Efficient Control of Crop Diseases.Plant biotechnology journal · 2026Article
- RNAi: the next wave of green agriculture, challenges, and bridging the translational gap.Plant cell reports · 2026Review
- Nano-enabled spray-induced RNA interference against fungal pathogens of oilseed crops: carrier performance, delivery evidence and field translation.World journal of microbiology & biotechnology · 2026Review
- Rational dsRNA design, scalable production and nanodelivery to enhance spray-induced gene silencing.Communications biology · 2026Review
- RNAI-based strategies and nanomaterial-mediated delivery for green control of clubroot disease in rapeseed.Journal of advanced research · 2026Article
- RNA interference in crop protection: opportunities and challenges during the transition to commercialization.Pest management science · 2026Review
- Nano-enabled RNAi strategies for sustainable citrus protection against viral pathogens.Planta · 2026Review
- RNAi Power Targets in Insect Pests: Beyond Functional Validation to Biopesticide Development Potential.Plants (Basel, Switzerland) · 2026Review
- A coordinated toolbox strategy integrating direct pathogen suppression, host-associated responses, and microbiome remodeling for crop protection.Journal of nanobiotechnology · 2026Article
- Extending bananas shelf life by dsRNA mediated interference of ethylene synthesis.Food chemistry. Molecular sciences · 2026Article
- LUCID: An Integrative Approach for Target Discovery and dsRNA Design in Plant Fungal Pathogens.Plant biotechnology journal · 2026Article
- The unfulfilled potential of nanocarriers for RNA delivery in antiviral crop protection.Nature plants · 2026Review
- Nanoclay-based dsRNA delivery: a novel approach to control Globodera rostochiensis.Molecular biology reports · 2026Article
- Nanomaterial-mediated delivery of tomato miR393 intrinsically targeting a cell wall-related gene of Botrytis cinerea confers efficient control of gray mold disease.Plant communications · 2026Article
- Cascaded regulatory network composed of small RNAs involves in the symbiosis of Panax notoginseng and fungus Acremonium sp. D212.Scientific reports · 2026Article
- Self-Assembling Nanoparticle Enhances RNA Interference Efficiency againstJournal of agricultural and food chemistry · 2026Article
- High-Efficiency and Eco-Friendly Management of Multiple Fungal Diseases by a Novel Carbon Dots sCDP Delivered RNA Nano-Pesticides.Plant biotechnology journal · 2026Article
- Plant-derived extracellular vesicles and nanoparticles: origins, functions, and applications.Frontiers in bioengineering and biotechnology · 2026Review
3 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 4 countries.
Funding
Abstract
Spray-induced gene silencing (SIGS) is an innovative and eco-friendly technology where topical application of pathogen gene-targeting RNAs to plant material can enable disease control. SIGS applications remain limited because of the instability of RNA, which can be rapidly degraded when exposed to various environmental conditions. Inspired by the natural mechanism of cross-kingdom RNAi through extracellular vesicle trafficking, we describe herein the use of artificial nanovesicles (AVs) for RNA encapsulation and control against the fungal pathogen, Botrytis cinerea. AVs were synthesized using three different cationic lipid formulations, DOTAP + PEG, DOTAP and DODMA, and examined for their ability to protect and deliver double stranded RNA (dsRNA). All three formulations enabled dsRNA delivery and uptake by B. cinerea. Further, encapsulating dsRNA in AVs provided strong protection from nuclease degradation and from removal by leaf washing. This improved stability led to prolonged RNAi-mediated protection against B. cinerea both on pre- and post-harvest plant material using AVs. Specifically, the AVs extended the protection duration conferred by dsRNA to 10 days on tomato and grape fruits and to 21 days on grape leaves. The results of this work demonstrate how AVs can be used as a new nanocarrier to overcome RNA instability in SIGS for crop protection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.