Evidence map›Paper›PMID 36600206›Full record

ArticleBMC genomics2023

Identification of biomarkers and therapeutic targets related to Sepsis-associated encephalopathy in rats by quantitative proteomics.

Miaoxian Yang, Yu He, Yuewen Xin, Junliang Jiang, Mi Tian, Jiaying Tan, Shuixiang Deng, Ye Gong

Open access · goldAbstract read
PubMed Publisher
In one paragraph

Article in BMC genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Miaoxian Yang *Department of Critical Care Medicine, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, 200040, Shanghai, China.
Yu He *Department of Critical Care Medicine, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, 200040, Shanghai, China.
Yuewen XinDepartment of Critical Care Medicine, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, 200040, Shanghai, China.
Junliang JiangDepartment of Critical Care Medicine, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, 200040, Shanghai, China.
Mi TianDepartment of Critical Care Medicine, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, 200040, Shanghai, China.
Jiaying TanDepartment of Critical Care Medicine, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, 200040, Shanghai, China.
Shuixiang DengDepartment of Critical Care Medicine, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, 200040, Shanghai, China. shuixiang2@126.com.
Ye GongDepartment of Critical Care Medicine, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, 200040, Shanghai, China. gong_ye@fudan.edu.cn.
Fudan University · CNHuashan Hospital · CN

Funding

National Natural Science Foundation of China 82072788Science and Technology Commission of Shanghai Municipality 19140900205Science and Technology Commission of Shanghai Municipality 21ZR1410700Shanghai Hospital Development Center SHDC2020CR3021A
6 · The paper itself

Abstract

backgroundSepsis-associated encephalopathy (SAE) is a common and severe complication of sepsis. While several studies have reported the proteomic alteration in plasma, urine, heart, etc. of sepsis, few research focused on the brain tissue. This study aims at discovering the differentially abundant proteins in the brains of septic rats to identify biomarkers of SAE.

methodsThe Prague-Dawley rats were randomly divided into sepsis (n = 6) or sham (n = 6) groups, and then the whole brain tissue was dissected at 24 h after surgery for further protein identification by Quantitative iTRAQ LC-MS/MS Proteomics. Ingenuity pathway analysis, Gene ontology knowledgebase, and STRING database are used to explore the biological significance of proteins with altered concentration.

resultsAmong the total of 3163 proteins identified in the brain tissue, 57 were increased while 38 were decreased in the sepsis group compared to the sham group. Bioinformatic analyses suggest that the differentially abundant proteins are highly related to cellular microtubule metabolism, energy production, nucleic acid metabolism, neurological disease, etc. Additionally, acute phase response signaling was possibly activated and PI3K/AKT signaling was suppressed during sepsis. An interaction network established by IPA revealed that Akt1, Gc-globulin, and ApoA1 were the core proteins. The increase of Gc-globulin and the decrease of Akt1 and ApoA1 were confirmed by Western blot.

conclusionBased on the multifunction of these proteins in several brain diseases, we first propose that Gc-globulin, ApoA1, PI3K/AKT pathway, and acute phase response proteins (hemopexin and cluster of alpha-2-macroglobulin) could be potential candidates for the diagnosis and treatment of SAE. These results may provide new insights into the pathologic mechanism of SAE, yet further research is required to explore the functional implications and clinical applications of the differentially abundant proteins in the brains of sepsis group.

Indexed as

GlobulinsProteomicsSepsis-Associated EncephalopathyAcute-Phase ReactionAnimalsBiomarkersChromatography, LiquidPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktRatsSepsisTandem Mass SpectrometryBiomarkersGlobulinsPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktAcute phase responseGCPI3K/AKT signalingQuantitative proteomicsSepsis-associated encephalopathy

Identifiers

PMID36600206
OpenAlexW4313477341

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.