Evidence map›Paper›PMID 36598925›Full record

ArticlePloS one2023

A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum.

Diana G Calvopina-Chavez, Robyn E Howarth, Audrey K Memmott, Oscar H Pech Gonzalez, Caleb B Hafen, Kyson T Jensen, Alex B Benedict, Jessica D Altman, Brittany S Burnside, Justin S Childs and 22 more

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.6field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Transcription Factor PecS MediatesJournal of bacteriology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

32 authors at 1 institution in 1 country.

Diana G Calvopina-ChavezDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.ORCID 0000-0001-6314-8895
Robyn E HowarthDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Audrey K MemmottDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Oscar H Pech GonzalezDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Caleb B HafenDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Kyson T JensenDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Alex B BenedictDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Jessica D AltmanDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Brittany S BurnsideDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Justin S ChildsDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Samuel W DallonDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Alexa C DeMarcoDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Kirsten C FlindtDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Sarah A GroverDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Elizabeth HeningerDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Christina S IversonDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Abigail K JohnsonDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Jack B LopezDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
McKay A MeinzerDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Brook A MoulderDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Rebecca I MoultonDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Hyrum S RussellDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Tiana M ScottDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Yuka ShiobaraDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Mason D TaylorDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Kathryn E TippetsDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Kayla M VainerereDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Isabella C Von WallwitzDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Madison WagleyDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Megumi S WileyDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Naomi J YoungDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Joel S GriffittsDepartment of Microbiology and Molecular Biology, Brigham Young University, Provo, UT, United States of America.
Brigham Young University · US

Funding

The structural basis of spatially constrained enzymatic promiscuityR15GM132852 · NIGMS · BRIGHAM YOUNG UNIVERSITY · PI GRIFFITTS, JOEL S · 2019 to 2019
$338k
NIGMS NIH HHS R15 GM132852
6 · The paper itself

Abstract

The genetic and molecular basis of flagellar motility has been investigated for several decades, with innovative research strategies propelling advances at a steady pace. Furthermore, as the phenomenon is examined in diverse bacteria, new taxon-specific regulatory and structural features are being elucidated. Motility is also a straightforward bacterial phenotype that can allow undergraduate researchers to explore the palette of molecular genetic tools available to microbiologists. This study, driven primarily by undergraduate researchers, evaluated hundreds of flagellar motility mutants in the Gram-negative plant-associated bacterium Agrobacterium fabrum. The nearly saturating screen implicates a total of 37 genes in flagellar biosynthesis, including genes of previously unknown function.

Indexed as

AgrobacteriumBacterial ProteinsFlagellaGene Expression Regulation, BacterialBacterial Proteins

Identifiers

PMID36598925
PMCPMC9812332
OpenAlexW4313485123

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.