Evidence map›Paper›PMID 36598700›Full record

ArticleFunctional & integrative genomics2023

Exosomal microRNA-342-5p from human umbilical cord mesenchymal stem cells inhibits preeclampsia in rats.

Yi Chen, Jiaxi Jin, XiaoPei Chen, Jia Xu, Lihong An, Haibo Ruan

Abstract read
PubMed Publisher
In one paragraph

Article in Functional & integrative genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
5.9field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 20 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Yi ChenDepartment of Obstetrics and Gynecology, The First People's Hospital of WenLing ZheJiang, No. 333, Chuanannan Road, Chengxi Street, Wenling, 317500, Zhejiang, China.
Jiaxi JinDepartment of Obstetrics and Gynecology, The First People's Hospital of WenLing ZheJiang, No. 333, Chuanannan Road, Chengxi Street, Wenling, 317500, Zhejiang, China.
XiaoPei ChenDepartment of Obstetrics and Gynecology, The First People's Hospital of WenLing ZheJiang, No. 333, Chuanannan Road, Chengxi Street, Wenling, 317500, Zhejiang, China.
Jia XuDepartment of Obstetrics and Gynecology, The First People's Hospital of WenLing ZheJiang, No. 333, Chuanannan Road, Chengxi Street, Wenling, 317500, Zhejiang, China.
Lihong AnDepartment of Obstetrics and Gynecology, The First People's Hospital of WenLing ZheJiang, No. 333, Chuanannan Road, Chengxi Street, Wenling, 317500, Zhejiang, China.
Haibo RuanDepartment of Obstetrics and Gynecology, The First People's Hospital of WenLing ZheJiang, No. 333, Chuanannan Road, Chengxi Street, Wenling, 317500, Zhejiang, China. Ruanhaibo3175@163.com.
The First People's Hospital of Wenling · CN

Funding

the study on the correlation between early spontaneous abortion and villus chromosome abnormality 2014C312068
6 · The paper itself

Abstract

We aimed to investigate the inhibitory effect of human umbilical cord mesenchymal stem cell (hucMSC)-derived exosomes (hucMSC-Exos) transmitting microRNA-342-5p (miR-342-5p) on the development of preeclampsia (PE) by targeting programmed cell death 4 (PDCD4). The primary hucMSCs were cultured and transfected with miR-342-5p, and the exosomes (Exo) were extracted from the hucMSCs. PE rats were performed with an intraperitoneal injection of L-NAME from days 11 to 19 of gestation, and injection of Exo, Exo-negative control (NC), Exo-miR-342-5p agomir, Exo-miR-342-5p antagomir, and overexpressing PDCD4 (oe-PDCD4) vector into the placenta on the 16th day of pregnancy. HE staining was utilized to observe the pathological changes in placental tissues. TUNEL staining was implemented to evaluate cell apoptosis in placental tissues. Blood pressure and 24-h urinary protein in pregnant rats were measured by a non-invasive rat tail artery blood pressure measurement and protein auto-analyzer. Expressions of miR-342-5p, PDCD4, proinflammatory cytokines (TNF-α and IL-1β), and anti-inflammatory cytokines (IL-10 and TGF-β) were detected by RT-qPCR, and PDCD4 protein expression was determined by Western blot. The interaction between miR-342-5p and PDCD4 was analyzed by luciferase activity assay. MiR-342-5p was downregulated while PDCD4 was upregulated in the placental tissues of PE rats. HucMSC-Exo relieved pathology and suppressed inflammatory response, and apoptosis in the placental tissues, as well as reducing blood pressure and 24-h urinary protein of PE rats. Elevated miR-342-5p enhanced the promoting influence of hucMSC-Exo on PE rats, while inhibited miR-342-5p reversed the functions of hucMSC-Exo on PE rats. miR-342-5p targeted PDCD4. Overexpression of PDCD4 worsened the development of PE in rats. HucMSC-Exo conveying elevated miR-342-5p inhibits the development of PE in a rat model through downregulating PDCD4.

Indexed as

Mesenchymal Stem CellsMicroRNAsPre-EclampsiaAnimalsApoptosis Regulatory ProteinsCytokinesFemaleHumansPlacentaPregnancyRatsRNA-Binding ProteinsUmbilical CordApoptosis Regulatory ProteinsCytokinesMicroRNAsMIRN342 microRNA, humanPDCD4 protein, humanPdcd4 protein, ratRNA-Binding ProteinsMesenchymal stem cell-derived exosomeMicroRNA-342-5pPreeclampsiaProgrammed cell death 4

Identifiers

PMID36598700
OpenAlexW4313546086

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.