ArticleScientific reports2023
Long-term taxonomic and functional stability of the gut microbiome from human fecal samples.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Early risk stratification of late-onset sepsis in very preterm infants by intestinal microbiota profiling: a multicenter case-control validation study.Gut microbes · 2026Article
- Review
- Associations of host gene expression in the brain, liver, muscle with fecal and cecal microbiota composition in pigs.Functional & integrative genomics · 2026Article
- Quantitative evaluation of microbiome sequencing resolution under varying experimental conditions using defined mock communities.Scientific reports · 2026Article
- Standardized and batch effect-independent technologies enable global collaboration in microbiome research.The ISME journal · 2026Article
- Characterisation of the gut-lung axis microbiome in clinically stable patients with chronic obstructive pulmonary disease.EBioMedicine · 2026Observational
- Next-Gen intestinal parasite detection: Leveraging metataxonomics for improved diagnosis of intestinal protists and helminths.PloS one · 2026Article
- Efficacy of oral and rectal administration of human faecal microbiota transplant (FMT) in human microbiota-associated mouse models.Scientific reports · 2025Article
- Animal Species and Identity Testing: Developments, Challenges, and Applications to Non-Human Forensics.Genes · 2025Review
- Neonatal microbiome in the multiomics era: development and its impact on long-term health.Pediatric research · 2025Review
- Comparative study of sample storage conditions on gut dysbiosis in peripheral artery disease.Research square · 2025Article
- A comprehensive atlas of the bonobo gut bacteriome and its associated host and exposome factors.Cell reports · 2025Article
- Field expedient stool collection methods for gut microbiome analysis in deployed military environments.mSphere · 2025Article
- Evaluating stool microbiome integrity after domestic freezer storage using whole-metagenome sequencing, genome assembly, and antimicrobial resistance gene analysis.Microbiology spectrum · 2025Article
- Evaluating long-term stool preservation methods for maximizing the recovery of viable human fecal microbiota.Gut microbes reports · 2025Article
- Assessment of the reproducibility of bacterial membrane vesicle isolation and characterization.Extracellular vesicles and circulating nucleic acids · 2025Article
- Stabilized and unstabilized sampling methods result in differential fecal 16S rRNA microbial sequencing results.PloS one · 2025Article
- Omnigene-GutAMB Express · 2024Article
- Intestinal Insights: The Gut Microbiome's Role in Atherosclerotic Disease: A Narrative Review.Microorganisms · 2024Review
- Comparison of shipping versus immediate freezer storage of vaginal samples for vaginal microbiota assessment.Sexually transmitted infections · 2024Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Appropriate storage of fecal samples is a critical step for unbiased analysis in human microbiome studies. The purpose of this study was to evaluate the stability of the fecal microbial community for up to 18 months. Ten healthy volunteers provided fecal samples at the Jeonbuk National University Hospital. Stool samples were stored under the following six conditions: four different storage temperatures (- 70 °C, - 20 °C, 4 °C, and room temperature [20-25 °C]) and two different collection tubes (OMNIgene-Gut and DNA/RNA shield-fecal collection tubes). The gut microbiome was analyzed with 16S rRNA sequencing. We compared the taxonomic composition, alpha diversity, beta diversity and inferred pathway abundance between the baseline and 18 months after storage. Samples collected in the DNA/RNA Shield-fecal collection tubes showed the best performance in preservation of the taxonomic composition at 18 months. Pairwise differences in alpha diversity metrics showed the least deviation from zero. The PERMANOVA test showed non-significant change of beta diversity metrics (Unweighted Unifrac: q-value 0.268; Weighted Unifrac: q-value 0.848). The functional stability was significantly well preserved in the DNA/RNA Shield-fecal collection tubes (adjusted p value < 0.05). Our results demonstrate the use of the DNA/RNA Shield-fecal collection tube as an alternative storage method for fecal samples to preserve the taxonomic and functional stability of the microbiome over a long term.
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