Evidence map›Paper›PMID 36596564›Full record

ArticleThe Journal of veterinary medical science2023

Investigation of dichlorodiphenyltrichloroethane (DDT) on xenobiotic enzyme disruption and metabolomic bile acid biosynthesis in DDT-sprayed areas using wild rats.

Kodai Motohira, Yared Beyene Yohannes, Yoshinori Ikenaka, Akifumi Eguchi, Shouta Mm Nakayama, Victor Wepener, Nico J Smit, Johan Hj VAN Vuren, Mayumi Ishizuka

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of veterinary medical science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.4field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 4 countries.

Kodai MotohiraLaboratory of Toxicology, Department of Environmental Veterinary Sciences, Laboratory of Toxicology, Hokkaido University, Hokkaido, Japan.
Yared Beyene YohannesLaboratory of Toxicology, Department of Environmental Veterinary Sciences, Laboratory of Toxicology, Hokkaido University, Hokkaido, Japan.
Yoshinori IkenakaLaboratory of Toxicology, Department of Environmental Veterinary Sciences, Laboratory of Toxicology, Hokkaido University, Hokkaido, Japan.
Akifumi EguchiCenter for Preventive Medical Sciences, Chiba University, Chiba, Japan.
Shouta Mm NakayamaLaboratory of Toxicology, Department of Environmental Veterinary Sciences, Laboratory of Toxicology, Hokkaido University, Hokkaido, Japan.
Victor WepenerWater Research Group, Unit for Environmental Sciences and Management, Potchefstroom Campus, North-West University, Potchefstroom, South Africa.
Nico J SmitWater Research Group, Unit for Environmental Sciences and Management, Potchefstroom Campus, North-West University, Potchefstroom, South Africa.
Johan Hj VAN VurenWater Research Group, Unit for Environmental Sciences and Management, Potchefstroom Campus, North-West University, Potchefstroom, South Africa.
Mayumi IshizukaLaboratory of Toxicology, Department of Environmental Veterinary Sciences, Laboratory of Toxicology, Hokkaido University, Hokkaido, Japan.
North-West University · ZAHokkaido University · JPChiba University · JPUniversity of Gondar · ETUniversity of Zambia · ZM

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dichlorodiphenyltrichloroethane (DDT) is an organochlorine insecticide used worldwide. Several studies have reported the toxic effects of DDT and its metabolites on steroid hormone biosynthesis; however, its environmental effects are not well understood. This study examined wild rats collected in DDT-sprayed areas of South Africa and quantified plasma metabolites using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS). Fold change analysis of the metabolome revealed the effect of DDT on bile acid biosynthesis. Gene expression of the related enzyme in rat liver samples was also quantified. Significant association was found between DDT and gene expression levels related to constitutive androstane receptor mediated enzymes, such as Cyp2b1 in rat livers. However, our results could not fully demonstrate that enzymes related to bile acid biosynthesis were strongly affected by DDT. The correlation between DDT concentration and gene expression involved in steroid hormone synthesis in testis was also evaluated; however, no significant correlation was found. The disturbance of metabolic enzymes occurred in rat liver in the target area. Our results suggest that DDT exposure affects gene expression in wild rats living in DDT-sprayed areas. Therefore, there is a need for DDT toxicity evaluation in mammals living in DDT-sprayed areas. We could not find an effective biomarker that could reflect the mechanism of DDT exposure; however, this approach can provide new insights for future research to evaluate DDT effects in sprayed areas.

Indexed as

DDTXenobioticsAnimalsAnimals, WildBile Acids and SaltsHormonesMaleMammalsRatsSteroidsBile Acids and SaltsDDTHormonesSteroidsXenobioticsbile acid biosynthesisdichlorodiphenyltrichloroethane (DDT)metabolomicssteroid hormone biosynthesiswild rat

Identifiers

PMID36596564
PMCPMC10017292
OpenAlexW4313441252

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.