Evidence map›Paper›PMID 36595947›Full record

ArticleSTAR protocols2022

Protocol to visualize distinct motoneuron pools in adult zebrafish via injection of retrograde tracers.

Maria Bertuzzi, Irene Pallucchi, Abdeljabbar El Manira

Abstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Maria BertuzziDepartment of Neuroscience, Karolinska Institutet, 171 77 Stockholm, Sweden.
Irene PallucchiDepartment of Neuroscience, Karolinska Institutet, 171 77 Stockholm, Sweden.
Abdeljabbar El ManiraDepartment of Neuroscience, Karolinska Institutet, 171 77 Stockholm, Sweden. Electronic address: abdel.elmanira@ki.se.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In adult zebrafish, slow, intermediate, and fast muscle fibers occupy distinct regions of the axial muscle, allowing the use of retrograde tracers for selective targeting of the motoneurons (MNs) innervating them. Here, we describe a protocol to label distinct MN pools and tissue processing for visualization with confocal laser microscopy. We outline the different steps for selective labeling of primary and secondary MNs together with spinal cord fixation, isolation, mounting, and imaging. For complete details on the use and execution of this protocol, please refer to Pallucchi et al. (2022)

Indexed as

Motor NeuronsZebrafishAnimalsInjectionsMusclesSpinal CordMicroscopyModel OrganismsMolecular/Chemical Probes

Identifiers

PMID36595947
PMCPMC9761367

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.