Evidence map›Paper›PMID 36595934›Full record

ArticleSTAR protocols2022

Protocol to identify and analyze mouse and human quiescent hematopoietic stem cells using flow cytometry combined with confocal imaging.

Jiajing Qiu, Vijay Menon, Nikolaos Tzavaras, Raymond Liang, Saghi Ghaffari

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Jiajing QiuDepartment of Cell, Developmental & Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Vijay MenonDepartment of Cell, Developmental & Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Nikolaos TzavarasDepartment of Neuroscience and Microscopy CoRE, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Raymond LiangDepartment of Cell, Developmental & Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Developmental and Stem Cell Biology Multidisciplinary Training, Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Saghi GhaffariDepartment of Cell, Developmental & Regenerative Biology, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Developmental and Stem Cell Biology Multidisciplinary Training, Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. Electronic address: saghi.ghaffari@mssm.edu.
Icahn School of Medicine at Mount Sinai · US

Funding

(PQ5) Mitochondria in Leukemic Stem Cell Disease ProgressionR01CA205975 · NCI · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI GHAFFARI, SAGHI · 2016 to 2025
$3.9M
Mitochondria in the Regulation of Terminal ErythropoiesisR01HL136255 · NHLBI · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI SAGHI GHAFFARI · 2017 to 2026
$3.8M
Interdisciplinary Training in Systems and Developmental Biology and Birth DefectsT32HD075735 · NICHD · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI JABS, ETHYLIN WANG · 2013 to 2022
$1.8M
Novel Determinants of Terminal Erythroid MaturationR01HL116365 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI BRESNICK, EMERY H, GHAFFARI, SAGHI · 2012 to 2015
$1.6M
Towards Understanding Molecular Mechanisms of Human Hematopoietic Stem Cells' QuiescenceR01HL161567 · NHLBI · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI GHAFFARI, SAGHI · 2022 to 2024
$1.0M
NCI NIH HHS R01 CA205975NHLBI NIH HHS R01 HL116365NHLBI NIH HHS R01 HL136255NHLBI NIH HHS R01 HL161567NICHD NIH HHS T32 HD075735
6 · The paper itself

Abstract

Mitochondrial membrane potential (MMP) segregates functionally distinct subsets within highly purified hematopoietic stem cells (HSCs). Here, we detail a protocol for FACS isolation of MMP sub-fractions of phenotypically defined mouse and human HSCs. These steps are followed by high-/super-resolution immunofluorescence microscopy of HSCs' lysosomes. While the protocol describes the isolation of quiescent HSCs, which are the most potent subsets, it could also be applied to other HSC subsets. This protocol overcomes some experimental challenges associated with low HSC numbers. For complete details on the use and execution of this protocol, please refer to Liang et al. (2020) and Qiu et al. (2021).

Indexed as

Hematopoietic Stem CellsAnimalsFlow CytometryHumansMiceCell BiologyCell cultureCell isolationFlow Cytometry/Mass CytometryMetabolismMicroscopyStem Cells

Identifiers

PMID36595934
PMCPMC9676629
OpenAlexW4309469997

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.