Evidence map›Paper›PMID 36595933›Full record

ArticleSTAR protocols2022

Protocol to map multi-transmitter neurons in the mouse brain using intersectional strategy.

Jian Xu, Anis Contractor, Yongling Zhu

Abstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. IFITM1/OVOL1 Axis Is a Novel Regulator of the Expansion of the Limbal Epithelial Stem/Early Transient Amplifying Cell Population.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jian XuDepartment of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
Anis ContractorDepartment of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
Yongling ZhuDepartment of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA; Department of Ophthalmology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. Electronic address: yongling-zhu@northwestern.edu.

Funding

Signal Processing in the Inner RetinaR01EY018204 · NEI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI DEVRIES, STEVEN H, ZHU, YONGLING · 2007 to 2024
$6.4M
The role of glutamate receptors in compulsive and perseverative behaviorR01MH099114 · NIMH · NORTHWESTERN UNIVERSITY AT CHICAGO · PI CONTRACTOR, ANIS · 2012 to 2022
$4.5M
Cell types and functional circuitry in the retinaR01EY030169 · NEI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI YONGLING ZHU · 2019 to 2026
$3.1M
Regulation of kainate receptor expression in cone bipolar cellsR01EY032506 · NEI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI CONTRACTOR, ANIS, DEVRIES, STEVEN H · 2022 to 2025
$1.7M
NEI NIH HHS R01 EY018204NEI NIH HHS R01 EY030169NEI NIH HHS R01 EY032506NIMH NIH HHS R01 MH099114
6 · The paper itself

Abstract

Although it is now known that certain neurons can produce, store, and release multiple neurotransmitters, their locations, abundance, and functions remain elusive. We developed intersectional genetic strategies to identify multi-transmitter neurons based on the expression of neurotransmitter-specific genes. Here we present our procedures for whole-brain mapping of GABA/glutamate co-releasing neurons. We also detail our technique for labeling GABA/glutamate neurons in specific brain regions with adeno-associated virus (AAV). Our protocol can be readily extended to other types of multi-transmitter neurons. For complete details on the use and execution of this protocol, please refer to Xu et al. (2022).

Indexed as

Glutamic AcidNeuronsAnimalsBraingamma-Aminobutyric AcidMicegamma-Aminobutyric AcidGlutamic AcidGeneticsNeuroscience

Identifiers

PMID36595933
PMCPMC9730222

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.