Evidence map›Paper›PMID 36595920›Full record

ArticleSTAR protocols2022

Labeling and analyzing lipid droplets in mouse muscle stem cells.

Jingjuan Chen, Feng Yue, Shihuan Kuang

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Jingjuan ChenDepartment of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA. Electronic address: chen2627@purdue.edu.
Feng YueDepartment of Animal Sciences, University of Florida, Gainesville, FL 32608, USA.
Shihuan KuangDepartment of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA. Electronic address: skuang@purdue.edu.
Purdue University West Lafayette · USUniversity of Florida · US

Funding

Immunomyoblasts in muscle regenerationR01AR078695 · NIAMS · PURDUE UNIVERSITY · PI KUANG, SHIHUAN · 2021 to 2025
$2.2M
Metabolic regulation of muscle satellite cell homeostasisR01DK132819 · NIDDK · PURDUE UNIVERSITY · PI Shihuan Kuang · 2023 to 2026
$2.0M
PTEN Function in Satellite CellsR01AR071649 · NIAMS · PURDUE UNIVERSITY · PI KUANG, SHIHUAN · 2017 to 2021
$1.7M
Targeting PTEN to ameliorate muscular dystrophy in a mouse modelR01AR079235 · NIAMS · PURDUE UNIVERSITY · PI KUANG, SHIHUAN · 2022 to 2025
$1.3M
6 · The paper itself

Abstract

Lipid droplets are emerging as an important and dynamic organelle whose metabolism controls stem cell behavior. Here we present a comprehensive protocol to visualize and quantify these organelles in mouse muscle satellite cells (MuSCs). This protocol includes steps for BODIPY/LipidSpot610 staining of freshly isolated MuSCs, in vitro cultured myoblasts, and single myofibers to label lipid droplets and subsequent analysis and quantification of fluorescence signals. This protocol can be modified to stain lipid droplets in other cell types of interest. For complete details on the use and execution of this protocol, please refer to Yue et al. (2022).

Indexed as

Lipid DropletsMuscle, SkeletalAnimalsFluorescenceMiceMuscle Fibers, SkeletalStem CellsCell BiologyCell cultureFlow Cytometry/Mass CytometryMicroscopyMolecular/Chemical ProbesStem Cells

Identifiers

PMID36595920
PMCPMC9679676
OpenAlexW4309562785

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.