Evidence map›Paper›PMID 36595911›Full record

ArticleSTAR protocols2022

Protocol for isolation and ATAC-seq library construction of zebrafish red blood cells.

Yanyan Ding, Feng Liu

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Yanyan DingState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Institute for Stem Cell and Regeneration, University of Chinese Academy of Sciences, Beijing, China; School of Life Sciences, Shandong University, Qingdao, China.
Feng LiuState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Institute for Stem Cell and Regeneration, University of Chinese Academy of Sciences, Beijing, China; School of Life Sciences, Shandong University, Qingdao, China. Electronic address: liuf@ioz.ac.cn.
Shandong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding chromatin dynamics in red blood cells (RBCs) is critical for exploring the differentiation process and homeostasis maintenance during erythropoiesis. Here, we describe a protocol for isolation of zebrafish erythrocytes labelled with gata1:dsRed by fluorescence-activated cell sorting. We detail steps for ATAC-seq library construction from the isolated RBCs and describe how to analyze the quality of the library. The library can then be used to assay genome-wide chromatin accessibility in these RBCs. For complete details on the use and execution of this protocol, please refer to Ding et al. (2021).

Indexed as

Chromatin Immunoprecipitation SequencingZebrafishAnimalsChromatinErythrocytesSequence Analysis, DNAChromatinCell BiologyDevelopmental biology

Identifiers

PMID36595911
PMCPMC9700001
OpenAlexW4310009796

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.