Evidence map›Paper›PMID 36595885›Full record

ArticleSTAR protocols2022

Processing and cryopreservation of human ureter tissues for single-cell and spatial transcriptomics assays.

Emily E Fink, Surbhi Sona, Byron H Lee, Angela H Ting

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Emily E FinkGenomic Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA. Electronic address: finke@ccf.org.
Surbhi SonaGenomic Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA; Department of Nutrition, Center for Proteomics and Bioinformatics, School of Medicine, Case Western Reserve University, Cleveland, OH 44106, USA.
Byron H LeeCardiovascular & Metabolic Sciences, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA; Glickman Urological & Kidney Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Angela H TingGenomic Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA. Electronic address: tinga@ccf.org.
Cleveland Clinic Lerner College of Medicine · USCleveland Clinic · US

Funding

High resolution transcriptome and gene regulatory mapping of human ureter and bladder across the lifespanU01DK131383 · NIDDK · CLEVELAND CLINIC LERNER COM-CWRU · PI LEE, BYRON H, TING, ANGELA H · 2021 to 2025
$2.4M
Bacterial Rogue Methyltransferases Inducing Human EpimutationsF32CA260774 · NCI · CLEVELAND CLINIC LERNER COM-CWRU · PI FINK, EMILY E · 2021 to 2022
$92k
NCI NIH HHS F32 CA260774
6 · The paper itself

Abstract

Characterizing the cellular heterogeneity of human ureter tissues using single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics provides a detailed atlas of cell types, signaling networks, and potential cell-cell cross talk underlying developmental and regenerative pathways. We describe an optimized protocol for generating, cryopreserving, and thawing single-cell suspensions from ureter tissues isolated post-cystectomy for scRNA-seq. In addition, we describe an optimized protocol for cryopreserving human ureter tissues for 10x Genomics Visium spatial gene expression platform. For complete details on the use and execution of this protocol, please refer to Fink et al. (2022).

Indexed as

TranscriptomeUreterBiological AssayCryopreservationGene Expression ProfilingHumansCell isolationSingle Cell

Identifiers

PMID36595885
PMCPMC9668730
OpenAlexW4309564097

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.