Evidence map›Paper›PMID 36595117›Full record

ArticleGlycoconjugate journal2023

Partial characterization of purified glycoprotein from nutshell of Arachis hypogea L. towards macrophage activation and leishmaniacidal activity.

Sujatha Srinivasan, Mamilla R Charan Raja, Amrita Kar, Aishwarya Ramasamy, Adithyan Jayaraman, Vellingiri Vadivel, Santanu Kar Mahapatra

Abstract read
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In one paragraph

Article in Glycoconjugate journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Sujatha SrinivasanDepartment of Biotechnology, School of Chemical and Biotechnology, SASTRA Deemed to be University, 613 401, Thanjavur, India.
Mamilla R Charan RajaDepartment of Biotechnology, School of Chemical and Biotechnology, SASTRA Deemed to be University, 613 401, Thanjavur, India.
Amrita KarDepartment of Biotechnology, School of Chemical and Biotechnology, SASTRA Deemed to be University, 613 401, Thanjavur, India.
Aishwarya RamasamyDepartment of Biotechnology, School of Chemical and Biotechnology, SASTRA Deemed to be University, 613 401, Thanjavur, India.
Adithyan JayaramanDepartment of Biotechnology, School of Chemical and Biotechnology, SASTRA Deemed to be University, 613 401, Thanjavur, India.
Vellingiri VadivelDepartment of Biotechnology, School of Chemical and Biotechnology, SASTRA Deemed to be University, 613 401, Thanjavur, India.
Santanu Kar MahapatraDepartment of Biotechnology, School of Chemical and Biotechnology, SASTRA Deemed to be University, 613 401, Thanjavur, India. sailar.santanu@gmail.com.ORCID http://orcid.org/0000-0002-2257-8538
SASTRA University · INMidnapore Medical College and Hospital · INSaveetha University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Arachis hypogea L. protein fraction-2 (AHP-F2) from the Peanut shell was extracted and characterized and its potent immunomodulatory and anti-leishmanial role was determined in this present study. AHP-F2 was found to be a glycoprotein as the presence of carbohydrates were confirmed by the analysis of high-performance liquid chromatography (HPLC) yielded glucose, galactose, mannose, and xylose. AHP-F2 molecular mass was found to be ∼28 kDa as indicated in MALDI-TOF and peptide mass fingerprinting analysis followed by Mascot search. The peptide matches revealed the similarity of the mannose/glucose binding lectin with 71.07% in the BLAST analysis. After that, the 3D structure of the AHP-F2 model was designed and validated by the Ramachandran plot. The immunomodulatory role of AHP-F2 was established in murine peritoneal macrophages as induction of nitric oxide (NO), and stimulation of proinflammatory cytokines (IL-12 and IFN-γ) in a dose-dependent manner was observed. Interestingly, it was also found that AHP-F2 has interacted with the innate immune receptor, toll-like receptors (TLRs) as established in molecular docking as well as mRNA expression. The anti-leishmanial potential of AHP-F2 was revealed with a prominent inhibition of amastigote growth within the murine macrophages with prompt induction of nitrite release. Altogether, the isolated AHP-F2 from Arachis hypogea L. has strong immunomodulatory and anti-leishmanial potential which may disclose a new path to treat leishmaniasis.

Indexed as

ArachisLeishmania donovaniAnimalsGlucoseGlycoproteinsMacrophage ActivationMannoseMiceMice, Inbred BALB CMolecular Docking SimulationNitric OxideGlucoseGlycoproteinsMannoseNitric OxideArachis hypogea L. nutshellGlycoproteinLeishmania donovaniMacrophage activationNitric oxide (NO)

Identifiers

PMID36595117
OpenAlexW4313433395

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.