Evidence map›Paper›PMID 36595058›Full record

ArticleActa neurochirurgica2023

Artificial cerebrospinal fluid restores aspirin-inhibited physiological hemostasis through recovery of platelet aggregation function.

Ryosuke Suzuki, Yukinori Kozuma, Chisako Inoue, Kano Tanabe, Ippei Noboruo, Hohomi Arao, Tatsuya Kawaguchi, Nobuyuki Shimizu, Tetsuya Yamamoto

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In one paragraph

Article in Acta neurochirurgica, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Ryosuke Suzuki *Department of Neurosurgery, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.
Yukinori Kozuma *Department of Medical Technology, Faculty of Health Science, Kumamoto Health Science University, Kumamoto, Kumamoto, Japan.
Chisako InoueDivision of Medical Sciences, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.
Kano TanabeDepartment of Medical Technology, Faculty of Health Science, Kumamoto Health Science University, Kumamoto, Kumamoto, Japan.
Ippei NoboruoDepartment of Medical Technology, Faculty of Health Science, Kumamoto Health Science University, Kumamoto, Kumamoto, Japan.
Hohomi AraoDepartment of Medical Technology, Faculty of Health Science, Kumamoto Health Science University, Kumamoto, Kumamoto, Japan.
Tatsuya KawaguchiDepartment of Medical Technology, Faculty of Health Science, Kumamoto Health Science University, Kumamoto, Kumamoto, Japan.
Nobuyuki ShimizuDepartment of Neurosurgery, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan.
Tetsuya YamamotoDepartment of Neurosurgery, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, Japan. y_neuros@yokohama-cu.ac.jp.
Kumamoto Health Science University · JPYokohama City University · JPUniversity of Tsukuba · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundOptimal hemostasis provides safety and reliability during neurosurgery which improves surgical outcomes. Previously, artificial cerebrospinal fluid (aCSF) and its component sodium bicarbonate were found to facilitate physiological hemostasis by amplifying platelet aggregation. This study aimed to verify whether aCSF amplifies platelet-dependent hemostasis in the presence of antiplatelet agents.

methodsWe prepared platelet-rich plasma (PRP) or washed platelets using aspirin (acetylsalicylic acid, (ASA)) or normal saline (NS). We evaluated samples treated with a commercially available aCSF solution or NS for amplification of aggregation, activation of integrin αIIbβ3, phosphatidylserine (PS) exposure, P-selectin (CD62P) expression, and formation of microparticles (MPs). We assessed the effect of aCSF on in vivo hemostasis in the presence of ASA by measuring the tail bleeding time in ASA-or NS-injected C57BL/6 N mice.

resultsCompared with NS, aCSF amplified ASA-inhibited platelet aggregation by recovering platelet activation including PS exposure, MP release, CD62P expression, and integrin αIIbβ3 activation. When using washed platelets, aCSF almost completely counteracted the inhibition of platelet aggregation by ASA. Prolonged bleeding time from the amputated tail of ASA-injected mice was significantly shortened by the treatment with aCSF compared to NS. Sodium bicarbonate also directly amplified ASA-inhibited platelet aggregation.

conclusionsaCSF and sodium bicarbonate facilitate physiological hemostasis through the recovery of inhibited platelet aggregation even in the presence of ASA. The utilization of aCSF in the operative field may be advantageous for facilitating hemostasis in patients with impaired platelet function and contribute to improving outcomes of neurosurgery.

Indexed as

AspirinPlatelet AggregationAnimalsBlood PlateletsHemostasisMiceMice, Inbred C57BLPlatelet Aggregation InhibitorsPlatelet Glycoprotein GPIIb-IIIa ComplexReproducibility of ResultsSodium BicarbonateAspirinPlatelet Aggregation InhibitorsPlatelet Glycoprotein GPIIb-IIIa ComplexSodium BicarbonateArtificial cerebrospinal fluidAspirinHemostasisPlatelet aggregationRecovery of functionSodium bicarbonate

Identifiers

PMID36595058
OpenAlexW4313446487

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.