ArticleNature biotechnology2023
Genomically mined acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria.
Article in Nature biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 126 citations in OpenAlex.
- Trimodal ultrasound imaging of brain-tumor interaction.iScience · 2026Article
- Accelerated immunostaining of thick biological tissues using bidirectional electric fields with reversible deformation.Biomedical optics express · 2026Article
- Engineered bacteria as exogenous organelle mimics restore PTEN and p53 tumor suppressor functions for cancer therapy.Nature communications · 2026Article
- Endothelial Continuum and Capillary Specialization in Pulmonary Vascular Development.Arteriosclerosis, thrombosis, and vascular biology · 2026Review
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- Multifiber Array-Based Photometry System for Multiregional Functional Mapping in the Mouse Brain.The European journal of neuroscience · 2026Article
- Monitoring in vivo transcription with synthetic serum markers.Nature communications · 2026Article
- Real-time volumetric imaging of cells and molecules in deep tissues with Takoyaki ultrasound.Nature communications · 2026Article
- Gas vesicle-expressing human pluripotent stem cells enable multimodal ultrasound and optical coherence tomographic imaging.BMC biotechnology · 2026Article
- An ultrasound-scanning in vivo light source.Nature materials · 2026Article
- Ultrasound-Responsive Engineered Bacteria for Targeted Cancer Therapy: Strategies, Mechanisms, and Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- LIFUS-driven engineered bacteria reprogram immunosuppressive niches via mechano-NOTCH signaling.Cell reports. Medicine · 2026Article
- Screening and Evaluation of Chassis Cells for Heterologous Biosynthesis of Gas Vesicles as Ultrasound Contrast Agents.Marine drugs · 2026Article
- Ultrasound-driven mechanophore activation in living plants.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Attenuated Bacteria-Based Tumor Therapy: Clinical Application Risks, Marketing Approval Restrictions, and Coping Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Bacteria as living biosensors for DNA.Nature reviews bioengineering · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Gas Vesicles and Acoustic Protein Nanostructures in Molecular Ultrasound Nanomedicine: Translational Archetypes, Biomaterial Design, and Barriers to Clinical Realization.International journal of nanomedicine · 2026Review
- Metabolic Gadolinium Labeling ofBiomaterials research · 2026Article
- The human microbiome reshapes the breast cancer immune-metabolic-hormonal microenvironment.Frontiers in immunology · 2026Review
12 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
Abstract
Ultrasound allows imaging at a much greater depth than optical methods, but existing genetically encoded acoustic reporters for in vivo cellular imaging have been limited by poor sensitivity, specificity and in vivo expression. Here we describe two acoustic reporter genes (ARGs)-one for use in bacteria and one for use in mammalian cells-identified through a phylogenetic screen of candidate gas vesicle gene clusters from diverse bacteria and archaea that provide stronger ultrasound contrast, produce non-linear signals distinguishable from background tissue and have stable long-term expression. Compared to their first-generation counterparts, these improved bacterial and mammalian ARGs produce 9-fold and 38-fold stronger non-linear contrast, respectively. Using these new ARGs, we non-invasively imaged in situ tumor colonization and gene expression in tumor-homing therapeutic bacteria, tracked the progression of tumor gene expression and growth in a mouse model of breast cancer, and performed gene-expression-guided needle biopsies of a genetically mosaic tumor, demonstrating non-invasive access to dynamic biological processes at centimeter depth.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.