Evidence map›Paper›PMID 36593026›Full record

ArticleIn vivo (Athens, Greece)

Blueberry Leaf Extract Prevents Lacrimal Hyposecretion in Sjögren's Syndrome-like Model of Non-obese Diabetic Mice.

Kenjirou Ogawa, Karin Urata, Saki Maeda, Yuta Ohno, Keitaro Satoh, Yoshiyuki Yamada, Yosuke Suzuki, Yasuko Koga, Kazuhiro Sugamoto, Makiko Kawaguchi and 5 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in In vivo (Athens, Greece). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Extract fromNutrients · 2024
    Trial
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

Kenjirou OgawaInstitute for Tenure Track Promotion, University of Miyazaki, Miyazaki, Japan; ogawa.kenjirou.u2@cc.miyazaki-u.ac.jp.
Karin UrataGraduate School of Agriculture, University of Miyazaki, Miyazaki, Japan.
Saki MaedaDepartment of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Yuta OhnoDepartment of Pharmacology, Asahi University School of Dentistry, Mizuho, Japan.
Keitaro SatohDepartment of Pharmacology, Meikai University School of Dentistry, Sakado, Japan.
Yoshiyuki YamadaDepartment of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki, Japan.
Yosuke SuzukiDepartment of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Miyazaki, Japan.
Yasuko KogaGraduate School of Agriculture, University of Miyazaki, Miyazaki, Japan.
Kazuhiro SugamotoDepartment of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Miyazaki, Japan.
Makiko KawaguchiDepartment of Pathology, Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
Hisato KunitakeGraduate School of Agriculture, University of Miyazaki, Miyazaki, Japan.
Kazuo NishiyamaGraduate School of Agriculture, University of Miyazaki, Miyazaki, Japan.
Y O GotoBiolabo Co., Ltd., Kobe, Japan.
Takayuki NakayamaBiolabo Co., Ltd., Kobe, Japan.
Masao YamasakiGraduate School of Agriculture, University of Miyazaki, Miyazaki, Japan.
University of Miyazaki · JPAsahi University · JPMeikai University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND/

aimThis study evaluated the effect of blueberry leaf hot water extract (BLEx) on Sjögren's syndrome (SS)-like lacrimal hyposecretion in male non-obese diabetic (NOD) mice. MATERIALS AND

methodsNOD or BALB/c mice were fed 1% BLEx or control (AIN-93G) for 2 weeks from the age of 4 to 6 weeks. Pilocarpine-induced tear volume was measured using a phenol red-impregnated thread. The lacrimal glands were evaluated histologically by H&E staining. The IL-1β and TNF-α levels in the lacrimal gland tissue were measured by ELISA. The mRNA expression levels of secretion-related proteins were measured by real-time PCR. LC3 I/II and arginase 1 expression levels were measured by western blot.

resultsAfter feeding with BLEx, pilocarpine-induced tear secretion in NOD mice was increased. In contrast, the mRNA expression levels of the cholinergic muscarinic M3 receptor, aquaporin 5, and ion channels related to lacrimal secretion were not changed by BLEx administration. In addition, the protein expression of arginase 1, which was recently reported to be involved in tear hyposecretion in NOD mice, was also not improved by BLEx administration. Although infiltration in the lacrimal gland of NOD mice was not decreased, the levels of TNF-α and the autophagy-related protein LC3 were significantly suppressed by BLEx treatment.

conclusionBLEx treatment may ameliorate lacrimal hyposecretion in NOD mice by delaying the progression of autoimmune disease by suppressing autophagy in lacrimal glands.

Indexed as

Blueberry PlantsDiabetes Mellitus, ExperimentalLacrimal ApparatusSjogren's SyndromeAnimalsArginaseDisease Models, AnimalMaleMiceMice, Inbred NODPilocarpinePlant ExtractsRNA, MessengerTumor Necrosis Factor-alphaArginasePilocarpinePlant ExtractsRNA, MessengerTumor Necrosis Factor-alphablueberry leafDry eyelacrimal glandproanthocyanidinSjögren’s syndrometear secretion

Identifiers

PMID36593026
PMCPMC9843763
OpenAlexW4313474632

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.