Evidence map›Paper›PMID 36589127›Full record

ArticleFrontiers in plant science2022

Knockout of phytoene desaturase gene using CRISPR/Cas9 in highbush blueberry.

Giuseppe Vaia, Vera Pavese, Andrea Moglia, Valerio Cristofori, Cristian Silvestri

Open access · goldAbstract read
In one paragraph

Article in Frontiers in plant science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.5field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 30 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
  5. CRISPR/Cas9-MediatedPlants (Basel, Switzerland) · 2025
    Article
  6. Review
  7. Article
  8. Efficient CRISPR/Frontiers in plant science · 2025
    Article
  9. Article
  10. Advances inFrontiers in plant science · 2024
    Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Giuseppe VaiaDipartimento di Scienze Agrarie e Forestali (DAFNE), Università della Tuscia, Viterbo, Italy.
Vera PaveseDipartimento di Scienze Agrarie, Forestali e Alimentari (DISAFA), Università degli Studi di Torino, Grugliasco, Italy.
Andrea MogliaDipartimento di Scienze Agrarie, Forestali e Alimentari (DISAFA), Università degli Studi di Torino, Grugliasco, Italy.
Valerio CristoforiDipartimento di Scienze Agrarie e Forestali (DAFNE), Università della Tuscia, Viterbo, Italy.
Cristian SilvestriDipartimento di Scienze Agrarie e Forestali (DAFNE), Università della Tuscia, Viterbo, Italy.
Università degli Studi della Tuscia · ITUniversity of Turin · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Among the New Plant Breeding Techniques (NPBTs), the CRISPR/Cas9 system represents a useful tool for target gene editing, improving the traits of the plants rapidly. This technology allows targeting one or more sequences simultaneously, as well as introducing new genetic variations by homology-directed recombination. However, the technology of CRISPR/Cas9 remains a challenge for some polyploid woody species, since all the different alleles for which the mutation is required must be simultaneously targeted. In this work we describe improved protocols adapting the CRISPR/Cas9 system to highbush blueberry (

Indexed as

de novo shoot organogenesisgene editinggene loss-of-functionphytoene desaturaseVaccinium corymbosum L.

Identifiers

PMID36589127
PMCPMC9800005
OpenAlexW4311515615

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.