ArticlePlant biotechnology journal2023
New semi-dwarfing alleles with increased coleoptile length by gene editing of gibberellin 3-oxidase 1 using CRISPR-Cas9 in barley (Hordeum vulgare L.).
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 30 citations in OpenAlex.
- Early Plant Development as a Systems-Level Trait: Integrating Omics, Artificial Intelligence, and Emerging Biotechnologies.Plants (Basel, Switzerland) · 2026Review
- Translating research on seed dormancy and germination from Arabidopsis to temperate cereals to control pre-harvest sprouting.Frontiers in plant science · 2026Review
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- Genome editing for grass improvement and future agriculture.Horticulture research · 2025Article
- Diversity of Gibberellin 2-oxidase genes in the barley genome offers opportunities for genetic improvement.Journal of advanced research · 2024Article
- Heat stress causes chromatin accessibility and related gene expression changes in crown tissues of barley (Hordeum vulgare).Plant molecular biology · 2024Article
- Genome editing of five starch synthesis genes produces highly resistant starch and dietary fibre in barley grains.Plant biotechnology journal · 2024Article
- Investigating the genetic control of plant development in spring barley under speed breeding conditions.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
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- Harnessing the potential of mutation breeding, CRISPR genome editing, and beyond for sustainable agriculture.Functional & integrative genomics · 2024Review
- A short stature allele enhances tolerance to zinc deficiency and translocation of zinc in barley.PeerJ · 2024Article
- Recent advances of CRISPR-based genome editing for enhancing staple crops.Frontiers in plant science · 2024Review
- Worldwide study on field trials of biotechnological crops: new promises but old policy hurdles.Frontiers in plant science · 2024Article
- Grain yield improvement by genome editing of TaARF12 that decoupled peduncle and rachis development trajectories via differential regulation of gibberellin signalling in wheat.Plant biotechnology journal · 2023Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The green revolution was based on genetic modification of the gibberellin (GA) hormone system with "dwarfing" gene mutations that reduces GA signals, conferring shorter stature, thus enabling plant adaptation to modern farming conditions. Strong GA-related mutants with shorter stature often have reduced coleoptile length, discounting yield gain due to their unsatisfactory seedling emergence under drought conditions. Here we present gibberellin (GA) 3-oxidase1 (GA3ox1) as an alternative semi-dwarfing gene in barley that combines an optimal reduction in plant height without restricting coleoptile and seedling growth. Using large-scale field trials with an extensive collection of barley accessions, we showed that a natural GA3ox1 haplotype moderately reduced plant height by 5-10 cm. We used CRISPR/Cas9 technology, generated several novel GA3ox1 mutants and validated the function of GA3ox1. We showed that altered GA3ox1 activities changed the level of active GA isoforms and consequently increased coleoptile length by an average of 8.2 mm, which could provide essential adaptation to maintain yield under climate change. We revealed that CRISPR/Cas9-induced GA3ox1 mutations increased seed dormancy to an ideal level that could benefit the malting industry. We conclude that selecting HvGA3ox1 alleles offers a new opportunity for developing barley varieties with optimal stature, longer coleoptile and additional agronomic traits.
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