Evidence map›Paper›PMID 36587136›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

3D Hydrogel Cultures for High-Throughput Drug Discovery.

Karen Sperle, Darrin J Pochan, Sigrid A Langhans

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Karen SperleNemours Biomedical Research, Nemours Children's Hospital - Delaware, Wilmington, DE, USA.
Darrin J PochanDepartment of Materials Science and Engineering, University of Delaware, Newark, DE, USA.
Sigrid A LanghansNemours Biomedical Research, Nemours Children's Hospital - Delaware, Wilmington, DE, USA. Sigrid.Langhans@nemours.org.

Funding

Subproject Title: Clinical Research Education, Mentoring and Career Development CoreU54GM104941 · NIGMS · UNIVERSITY OF DELAWARE · PI Megan M Wenner · 2013 to 2026
$60.5M
Targeting mechanosignaling in pediatric brain cancerR01CA263216 · NCI · NEMOURS CHILDREN'S HOSPITAL, DELAWARE · PI Sigrid A Langhans · 2022 to 2026
$1.6M
NCI NIH HHS R01 CA263216NIGMS NIH HHS U54 GM104941
6 · The paper itself

Abstract

Our increased understanding of how a cell's microenvironment influences its behavior has fueled an interest in three-dimensional (3D) cell cultures for drug discovery. Particularly, scaffold-based 3D cultures are expected to recapitulate in vivo tissue stiffness and extracellular matrix composition more accurately than standard two-dimensional (2D) monolayer cultures. Here we present a 3D hydrogel cell culture setup suitable for automated screening with standard high-throughput screening (HTS) liquid handling equipment commonly found in a drug discovery laboratory. Further, we describe the steps required to validate the assay system for compound screening.

Indexed as

Drug DiscoveryHydrogelsCell Culture TechniquesExtracellular MatrixHigh-Throughput Screening AssaysHydrogels3D cultureExtracellular matrixHigh-throughput screeningHydrogelLiquid handlingScaffoldStiffness

Identifiers

PMID36587136
PMCPMC10786336

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.