Evidence map›Paper›PMID 36585836›Full record

ArticleProtein science : a publication of the Protein Society2023

High-yield recombinant bacterial expression of

Garry W Buchko, Mowei Zhou, Cat Hoang Vesely, Jinhui Tao, Wendy J Shaw, Ryan A Mehl, Richard B Cooley

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein science : a publication of the Protein Society, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. High-yield recombinant bacterial expression ofProtein science : a publication of the Protein Society · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Garry W BuchkoEarth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA.ORCID 0000-0002-3639-1061
Mowei ZhouEarth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA.ORCID 0000-0003-3575-3224
Cat Hoang VeselyDepartment of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon, USA.
Jinhui TaoPhysical and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA.
Wendy J ShawPhysical and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, Washington, USA.ORCID 0000-0002-4696-7415
Ryan A MehlDepartment of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon, USA.
Richard B CooleyDepartment of Biochemistry and Biophysics, Oregon State University, Corvallis, Oregon, USA.
Pacific Northwest National Laboratory · USOregon State University · US

Funding

Structural, spatial, and temporal features guiding amelogenins transformation of calcium phosphate into enamelR01DE015347 · NIDCR · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI SHAW, WENDY J · 2005 to 2025
$8.2M
NIDCR NIH HHS R01 DE015347
6 · The paper itself

Abstract

Amelogenin constitutes ~90% of the enamel matrix in the secretory stage of amelogenesis, a still poorly understood process that results in the formation of the hardest and most mineralized tissue in vertebrates-enamel. Most biophysical research with amelogenin uses recombinant protein expressed in Escherichia coli. In addition to providing copious amounts of protein, recombinant expression allows

Indexed as

AmelogeninEscherichia coliGenetic CodeRecombinant ProteinsSerineAnimalsMicePhosphoserineAmelogeninAmelx protein, mousePhosphoserineRecombinant ProteinsSerine31P-NMRamelogenesisamelogeninbiomineralizationisotope labelingnanospheresphosphoproteinsposttranslational modificationsrecombinant protein expression

Identifiers

PMID36585836
PMCPMC9850436
OpenAlexW4313340272

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.