ArticleProtein science : a publication of the Protein Society2023
Live-cell super-resolution imaging of actin using LifeAct-14 with a PAINT-based approach.
Article in Protein science : a publication of the Protein Society, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy.Nano letters · 2026Article
- A single-molecule reporter of membrane-proximal actin detects rapid remodeling upon B cell receptor clustering.bioRxiv : the preprint server for biology · 2026Article
- Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy.bioRxiv : the preprint server for biology · 2026Article
- Establishing functional giant Dictyostelium cells reveals front-rear polarity in intracellular signaling.Communications biology · 2026Article
- Effect of Retinal on Dictyostelium Cells During Development.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
- Phalloidin-PAINT: Enhanced quantitative nanoscale imaging of F-actin.Biophysical journal · 2024Article
- Quantitative Superresolution Imaging of F-Actin in the Cell Body and Cytoskeletal Protrusions Using Phalloidin-Based Single-Molecule Labeling and Localization Microscopy.bioRxiv : the preprint server for biology · 2024Article
- Imaging Proteins Sensitive to Direct Fusions Using Transient Peptide-Peptide Interactions.Nano letters · 2023Article
- Live-cell super-resolution imaging of actin using LifeAct-14 with a PAINT-based approach.Protein science : a publication of the Protein Society · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
We present direct-LIVE-PAINT, an easy-to-implement approach for the nanoscopic imaging of protein structures in live cells using labeled binding peptides. We demonstrate the feasibility of direct-LIVE-PAINT with an actin-binding peptide fused to EGFP, the location of which can be accurately determined as it transiently binds to actin filaments. We show that direct-LIVE-PAINT can be used to image actin structures below the diffraction-limit of light and have used it to observe the dynamic nature of actin in live cells. We envisage a similar approach could be applied to imaging other proteins within live mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.