ArticleJournal of advanced research2023
Identification of oxidosqualene cyclases associated with saponin biosynthesis from Astragalus membranaceus reveals a conserved motif important for catalytic function.
Article in Journal of advanced research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- Diverse oxidosqualene cyclases for triterpenoid biosynthesis.Synthetic and systems biotechnology · 2027Review
- Integrated transcriptomic and metabolomic analysis unveils biosynthetic mechanisms of key bioactive isoflavonoid accumulation inHorticulture research · 2026Article
- Elucidating the mechanisms underlying differential anthocyanin biosynthesis and its link to stem color and root isoflavonoid levels inHorticulture research · 2026Article
- The potential of metabolic engineering for sustainable phytosterol production.Advanced biotechnology · 2026Review
- Metabolic engineering of Phaeodactylum tricornutum for heterologous production of the plant triterpenoid friedelin.Microbial cell factories · 2025Article
- Identification of 2,3-oxidosqualene cyclase gene inHorticulture research · 2025Article
- Plant-derived triterpenoid saponins: multifaceted roles and bioengineering prospects.Plant cell reports · 2025Review
- Macrophage polarization in disease therapy: insights from astragaloside IV and cycloastragenol.Frontiers in pharmacology · 2025Review
- Total biosynthesis of the medicinal triterpenoid saponin astragalosides.Nature plants · 2024Article
- Identification and Expression Analysis of Cytochrome P450 Genes Probably Involved in Triterpenoid Saponins Biosynthesis inInternational journal of molecular sciences · 2024Article
- Research Progress on the Anti-Cancer Effects ofMolecules (Basel, Switzerland) · 2024Review
- Functional Identification ofInternational journal of molecular sciences · 2024Article
- Integrated Transcriptomics and Metabolomics Analysis Reveals the Effects of Cutting on the Synthesis of Flavonoids and Saponins in Chinese Herbal MedicineMetabolites · 2024Article
- MeJA-induced hairy roots inFrontiers in plant science · 2024Article
- Characterization and structure-based protein engineering of a regiospecific saponin acetyltransferase from Astragalus membranaceus.Nature communications · 2023Article
- Biosynthesis and Pharmacological Activities of Flavonoids, Triterpene Saponins and Polysaccharides Derived fromMolecules (Basel, Switzerland) · 2023Review
- Advances in Biotechnological Production and Metabolic Regulation ofPlants (Basel, Switzerland) · 2023Review
- Characterization and protein engineering of glycosyltransferases for the biosynthesis of diverse hepatoprotective cycloartane-type saponins in Astragalus membranaceus.Plant biotechnology journal · 2023Article
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Authors and funding
14 authors at 3 institutions in 2 countries.
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Abstract
introductionTriterpenoids and saponins have a broad range of pharmacological activities. Unlike most legumes which contain mainly oleanane-type scaffold, Astragalus membranaceus contains not only oleanane-type but also cycloartane-type saponins, for which the biosynthetic pathways are unknown.
objectivesThis work aims to study the function and catalytic mechanism of oxidosqualene cyclases (OSCs), one of the most important enzymes in triterpenoid biosynthesis, in A. membranaceus.
methodsTwo OSC genes, AmOSC2 and AmOSC3, were cloned from A. membranaceus. Their functions were studied by heterologous expression in tobacco and yeast, together with in vivo transient expression and virus-induced gene silencing. Site-directed mutagenesis and molecular docking were used to explain the catalytic mechanism for the conserved motif.
resultsAmOSC2 is a β-amyrin synthase which showed higher expression levels in underground parts. It is associated with the production of β-amyrin and soyasaponins (oleanane-type) in vivo. AmOSC3 is a cycloartenol synthase expressed in both aerial and underground parts. It is related to the synthesis of astragalosides (cycloartane-type) in the roots, and to the synthesis of cycloartenol as a plant sterol precursor. From AmOSC2/3, conserved triad motifs VFM/VFN were discovered for β-amyrin/cycloartenol synthases, respectively. The motif is a critical determinant of yield as proved by 10 variants from different OSCs, where the variant containing the conserved motif increased the yield by up to 12.8-fold. Molecular docking and mutagenesis revealed that Val, Phe and Met residues acted together to stabilize the substrate, and the cation-π interactions from Phe played the major role.
conclusionThe study provides insights into the biogenic origin of oleanane-type and cycloartane-type triterpenoids in Astragalus membranaceus. The conserved motif offers new opportunities for OSC engineering.
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