ArticleJournal of advanced research2023
A female-biased odorant receptor tuned to the lepidopteran sex pheromone in parasitoid Microplitis mediator guiding habitat of host insects.
Article in Journal of advanced research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 31 citations in OpenAlex.
- Eavesdropping Kairomone Markers of Interspecific Communication in Predatory and Parasitic Arthropods: A Review.Journal of chemical ecology · 2026Review
- Volatiles from Momordica charantia L. fruits and leaves attract Zeugodacus cucurbitae (Coquillett).Pest management science · 2026Article
- Molecular Recognition of Odorant-Binding Proteins WmagOBP39280 and WmagOBP42531 to Wohlfahrtia magnifica Pheromones and Bactrian Camel Volatiles.Archives of insect biochemistry and physiology · 2026Article
- Microbial Odorant Detection Guides Drosophila Parasitoids Seeking Hosts in Fermenting Fruits.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Function and Mechanism of ZcucOBP14 in Regulating Olfactory Recognition and Insecticide Susceptibility inInternational journal of molecular sciences · 2026Article
- Harnessing semiochemicals for parasitoid-based biological control: from laboratory identification to field applications.Crop health · 2025Review
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- Antenna-Biased Odorant Receptor PstrOR17 Mediates Attraction ofInternational journal of molecular sciences · 2024Article
- Identification and Comparative Expression Profiles of Candidate Olfactory Receptors in the Transcriptomes of the Important Egg Parasitoid WaspPlants (Basel, Switzerland) · 2023Article
- Sex pheromone communication in an insect parasitoid,Proceedings of the National Academy of Sciences of the United States of America · 2022Article
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Authors and funding
8 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe parasitoid wasp Microplitis mediator is an important natural enemy of the turnip moth Agrotis segetum and other Noctuidae pests. In our field observation, it was fortuitously discovered that sex pheromone traps used for A. segetum also attract female wasps, verified by a simulated field condition dual-choice laboratory assay. Therefore, it was hypothesized that olfactory recognition could be crucial in this process. In this regard, a female-biased odorant receptor of the wasp, MmedOR49, attracted our attention.
objectivesTo unravel the significance of the female-biased MmedOR49 regulating host pheromone recognition.
methodsExpression analysis (fluorescence in situ hybridization; quantitative realtime PCR), in vitro (two-electrode voltage-clamp recordings) and in vivo (RNAi combined with behavioral assessments) functional studies, and bioinformatics (structural modeling and molecular docking) were carried out to investigate the characteristics of MmedOR49.
resultsMmedOR49 expression was detected in the antennae of females by FISH. Quantification indicated that the expression level of MmedOR49 increased significantly after adult emergence. In vitro functional study revealed that MmedOR49 was specifically tuned to cis-5-decenyl acetate (Z5-10:Ac), the major sex pheromone component of A. segetum. Molecular docking showed that Z5-10:Ac strongly bound to the key amino acid residues His 80, Ile 81, and Arg 84 of MmedOR49 through hydrogen bonding. Behavioral assays indicated that female wasps were significantly attracted by Z5-10:Ac in a three-cage olfactometer. RNAi targeting further confirmed that MmedOR49 was necessary to recognize Z5-10:Ac, as female wasps lost their original behavioral responses to Z5-10:Ac after down-regulation of the MmedOR49 transcript.
conclusionAlthough M. mediator is a larval endoparasitoid, female wasps have a behavioral preference for a sex pheromone component of lepidopteran hosts. In this behavior, for female M. mediator, MmedOR49 plays an important role in guiding the habitat of host insects. These data provide a potential target for enhancing natural enemy utilization and pest control.
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