ArticleNucleic acids research2023
High-throughput quantitative binding analysis of DNA aptamers using exonucleases.
Article in Nucleic acids research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- High-Throughput Aptamer Characterization via Real-Time Nuclease Digestion.Journal of the American Chemical Society · 2026Article
- Article
- Modern Methods for Detection of Fentanyl and Its Analogues: A Comprehensive Review of Technologies and Applications.Molecules (Basel, Switzerland) · 2025Review
- Dissociation Constant (KSmall methods · 2025Review
- Exploring the relationship between aptamer binding thermodynamics, affinity, and specificity.Nucleic acids research · 2025Article
- Enhanced Sensitivity of Cell Identification in Complex Environments Using Chirally Inverted L-DNA-Based Logic Devices.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Nanotherapeutics for Alleviating Anesthesia-Associated Complications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Developing Aptamer-Based Colorimetric Opioid Tests.JACS Au · 2024Article
- Based on mutated aptamer-smartphone colorimetric detection of metronidazole in milk.Frontiers in bioengineering and biotechnology · 2024Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Aptamers are nucleic acid bioreceptors that have been used in various applications including medical diagnostics and as therapeutic agents. Identifying the most optimal aptamer for a particular application is very challenging. Here, we for the first time have developed a high-throughput method for accurately quantifying aptamer binding affinity, specificity, and cross-reactivity via the kinetics of aptamer digestion by exonucleases. We demonstrate the utility of this approach by isolating a set of new aptamers for fentanyl and its analogs, and then characterizing the binding properties of 655 aptamer-ligand pairs using our exonuclease digestion assay and validating the results with gold-standard methodologies. These data were used to select optimal aptamers for the development of new sensors that detect fentanyl and its analogs in different analytical contexts. Our approach dramatically accelerates the aptamer characterization process and streamlines sensor development, and if coupled with robotics, could enable high-throughput quantitative analysis of thousands of aptamer-ligand pairs.
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Registered trials
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