Evidence map›Paper›PMID 36582221›Full record

ArticleFrontiers in immunology2022

Engineered M2a macrophages for the treatment of osteoarthritis.

Chi Liang, Song Wu, Guang Xia, Junjie Huang, Zi Wen, Wenxiu Zhang, Xu Cao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
2.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.

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  16. The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in Osteoarthritis.Medical science monitor : international medical journal of experimental and clinical research · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Chi LiangDepartment of Orthopaedics of the 3rd Xiangya Hospital, Central South University, Changsha, China.
Song WuDepartment of Orthopaedics of the 3rd Xiangya Hospital, Central South University, Changsha, China.
Guang XiaDepartment of Orthopaedics of the 3rd Xiangya Hospital, Central South University, Changsha, China.
Junjie HuangDepartment of Orthopaedics of the 3rd Xiangya Hospital, Central South University, Changsha, China.
Zi WenDepartment of Orthopaedics of the 3rd Xiangya Hospital, Central South University, Changsha, China.
Wenxiu ZhangDepartment of Orthopaedics of the 3rd Xiangya Hospital, Central South University, Changsha, China.
Xu CaoDepartment of Orthopaedics of the 3rd Xiangya Hospital, Central South University, Changsha, China.
Xiangya Hospital Central South University · CNCentral South University · CNChinese Academy of Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Macrophage is a central regulator of innate immunity. Its M2 subsets, such as interstitial synovial macrophages, have been found to play critical roles in suppressing chronic inflammation and maintaining homeostasis within the joint. These macrophages have great potential as a disease-modifying cell therapy for osteoarthritis (OA). However, this has not yet been studied. Methods: Macrophages were isolated from the bone marrow of rats. We constructed a stable macrophage that "locked" in anti-inflammatory and pro-regenerative M2a polarity (L-M2a) by simultaneously knocking out tumor necrosis factor receptor 1 (TNFR1) and overexpressing IL-4 using Cas9-ribonuclear proteins (Cas9-RNP) and electroporation. Results: L-M2a macrophages displayed a typical anti-inflammatory phenotype similar to that of M2 macrophages Conclusion: The engineered L-M2a macrophages maintained a superior anti-inflammatory and pro-regenerative capacity in the inflammatory OA microenvironment and represents an ideal new strategy for the disease-modifying therapy of OA.

Indexed as

MacrophagesOsteoarthritisAnimalsCartilageInflammationRatsSynovial FluidCas9-ribonuclear proteinscell therapymacrophagesosteoarthritistumor necrosis factor receptor 1

Identifiers

PMID36582221
PMCPMC9792488
OpenAlexW4311219742

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.