ArticleProceedings of the National Academy of Sciences of the United States of America2023
Phosphates form spectroscopically dark state assemblies in common aqueous solutions.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- Water-Mediated Phosphoryl Wires Stabilize Pathological Tau Fibrils.Angewandte Chemie (International ed. in English) · 2026Article
- Aggregation of Phosphates Enhances Enzyme Activity in Aqueous Solution.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Classical Density Functional Treatment of Polydisperse Polarizable Clusters.The journal of physical chemistry. B · 2026Article
- Hyperpolarized NMR Combined with Quantum Mechanical Simulations Reveal Atomistic Structures of Calcium Phosphate Prenucleation Clusters.Analytical chemistry · 2025Article
- Evidence for a possible quantum effect on the formation of lithium-doped amorphous calcium phosphate from solution.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Characterization of Gramicidin A in Triblock and Diblock Polymersomes and Hybrid Vesicles via Continuous Wave Electron Paramagnetic Resonance Spectroscopy.Biomimetics (Basel, Switzerland) · 2025Article
- Quantum Models of Consciousness from a Quantum Information Science Perspective.Entropy (Basel, Switzerland) · 2025Article
- Hyperspectral reflectance imaging for visualizing reducing sugar content, moisture, and hollow rate in red ginseng.Heliyon · 2024Article
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- Conformational Landscape of NADH and Ion Binding in Water/DMSO Mixtures viaThe journal of physical chemistry. B · 2024Article
- Dynamic Nuclear Polarization Enhanced Multiple-Quantum Spin Counting of Molecular Assemblies in Vitrified Solutions.The journal of physical chemistry letters · 2024Article
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- Clinical research progress of callisperesDiscover oncology · 2024Review
- Luteolin alleviates cerebral ischemia/reperfusion injury by regulating cell pyroptosis.Open medicine (Warsaw, Poland) · 2024Article
- Biphasic NMR of Hyperpolarized Suspensions-Real-Time Monitoring of Solute-to-Solid Conversion to Watch Materials Grow.The journal of physical chemistry. C, Nanomaterials and interfaces · 2023Article
- Sodium Hexametaphosphate Serves as an Inducer of Calcium Signaling.Biomolecules · 2023Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphates and polyphosphates play ubiquitous roles in biology as integral structural components of cell membranes and bone, or as vehicles of energy storage via adenosine triphosphate and phosphocreatine. The solution phase space of phosphate species appears more complex than previously known. We present nuclear magnetic resonance (NMR) and cryogenic transmission electron microscopy (cryo-TEM) experiments that suggest phosphate species including orthophosphates, pyrophosphates, and adenosine phosphates associate into dynamic assemblies in dilute solutions that are spectroscopically "dark." Cryo-TEM provides visual evidence of the formation of spherical assemblies tens of nanometers in size, while NMR indicates that a majority population of phosphates remain as unassociated ions in exchange with spectroscopically invisible assemblies. The formation of these assemblies is reversibly and entropically driven by the partial dehydration of phosphate groups, as verified by diffusion-ordered spectroscopy (DOSY), indicating a thermodynamic state of assembly held together by multivalent interactions between the phosphates. Molecular dynamics simulations further corroborate that orthophosphates readily cluster in aqueous solutions. This study presents the surprising discovery that phosphate-containing molecules, ubiquitously present in the biological milieu, can readily form dynamic assemblies under a wide range of commonly used solution conditions, highlighting a hitherto unreported property of phosphate's native state in biological solutions.
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