Evidence map›Paper›PMID 36578046›Full record

ArticleArthritis research & therapy2022

Early removal of the infrapatellar fat pad/synovium complex beneficially alters the pathogenesis of moderate stage idiopathic knee osteoarthritis in male Dunkin Hartley guinea pigs.

Maryam F Afzali, Lauren B Radakovich, Madeline M Sykes, Margaret A Campbell, Kayley M Patton, Joseph L Sanford, Nicole Vigon, Ryan Ek, Gerardo E Narez, Angela J Marolf and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arthritis research & therapy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Maryam F AfzaliDepartment of Microbiology, Immunology and Pathology, Colorado State University, 200 West Lake Street, Fort Collins, CO, 80523, USA.
Lauren B RadakovichDepartment of Microbiology, Immunology and Pathology, Colorado State University, 200 West Lake Street, Fort Collins, CO, 80523, USA.
Madeline M SykesDepartment of Microbiology, Immunology and Pathology, Colorado State University, 200 West Lake Street, Fort Collins, CO, 80523, USA.
Margaret A CampbellDepartment of Microbiology, Immunology and Pathology, Colorado State University, 200 West Lake Street, Fort Collins, CO, 80523, USA.
Kayley M PattonDepartment of Microbiology, Immunology and Pathology, Colorado State University, 200 West Lake Street, Fort Collins, CO, 80523, USA.
Joseph L SanfordDepartment of Microbiology, Immunology and Pathology, Colorado State University, 200 West Lake Street, Fort Collins, CO, 80523, USA.
Nicole VigonDepartment of Biomedical Engineering, S631 Life Sciences Laboratory, University of Massachusetts, Amherst, 240 Thatcher Road, Amherst, MA, 01003, USA.
Ryan EkDepartment of Biomedical Engineering, S631 Life Sciences Laboratory, University of Massachusetts, Amherst, 240 Thatcher Road, Amherst, MA, 01003, USA.
Gerardo E NarezDepartment of Biomedical Engineering, S631 Life Sciences Laboratory, University of Massachusetts, Amherst, 240 Thatcher Road, Amherst, MA, 01003, USA.
Angela J MarolfDepartment of Environmental and Radiological Health Sciences, Colorado State University, 123 Flint Cancer Center, Fort Collins, CO, 80523, USA.
Katie J SikesDepartment of Clinical Sciences, Colorado State University, 1678 Clinical Sciences, Fort Collins, CO, 80523, USA.
Tammy L Haut DonahueBiomedical Engineering Department, The University of Memphis, 3806 Norriswood, Memphis, TN, 38152, USA.
Kelly S SantangeloDepartment of Microbiology, Immunology and Pathology, Colorado State University, 200 West Lake Street, Fort Collins, CO, 80523, USA. Kelly.Santangelo@colostate.edu.
Colorado State University · USUniversity of Massachusetts Amherst · USUniversity of Memphis · US

Funding

Biomedical Research Training for VeterinariansT32OD010437 · OD · COLORADO STATE UNIVERSITY · PI Gregg A Dean, KELLY S SANTANGELO · 2012 to 2026
$6.2M
Early removal of the infrapatellar fat pad as a novel treatment for osteoarthritisR21AR073972 · NIAMS · COLORADO STATE UNIVERSITY · PI SANTANGELO, KELLY S · 2019 to 2020
$381k
NIAMS NIH HHS R21 AR073972NIH HHS T32 OD010437
6 · The paper itself

Abstract

backgroundThe infrapatellar fat pad (IFP) is the largest adipose deposit in the knee; however, its contributions to the homeostasis of this organ remain undefined. To determine the influence of the IFP and its associated synovium (IFP/synovium complex or IFP/SC) on joint health, this study evaluated the progression of osteoarthritis (OA) following excision of this unit in a rodent model of naturally-occurring disease.

methodsMale Dunkin-Hartley guinea pigs (n=18) received surgical removal of the IFP in one knee at 3 months of age; contralateral knees received sham surgery as matched internal controls. Mobility and gait assessments were performed prior to IFP/SC removal and monthly thereafter. Animals were harvested at 7 months of age. Ten set of these knees were processed for microcomputed tomography (microCT), histopathology, transcript expression analyses, and immunohistochemistry (IHC); 8 sets of knees were dedicated to microCT and biomechanical testing (material properties of knee joints tissues and anterior drawer laxity).

resultsFibrous connective tissue (FCT) developed in place of the native adipose depot. Gait demonstrated no significant differences between IFP/SC removal and contralateral hindlimbs. MicroCT OA scores were improved in knees containing the FCT. Quantitatively, IFP/SC-containing knees had more osteophyte development and increased trabecular volume bone mineral density (vBMD) in femora and tibiae. Histopathology confirmed maintenance of articular cartilage structure, proteoglycan content, and chondrocyte cellularity in FCT-containing knees. Transcript analyses revealed decreased expression of adipose-related molecules and select inflammatory mediators in FCTs compared to IFP/SCs. This was verified via IHC for two key inflammatory agents. The medial articular cartilage in knees with native IFP/SCs showed an increase in equilibrium modulus, which correlated with increased amounts of magnesium and phosphorus. DISCUSSION/

conclusionFormation of the FCT resulted in reduced OA-associated changes in both bone and cartilage. This benefit may be associated with: a decrease in inflammatory mediators at transcript and protein levels; and/or improved biomechanical properties. Thus, the IFP/SC may play a role in the pathogenesis of knee OA in this strain, with removal prior to disease onset appearing to have short-term benefits.

Indexed as

Osteoarthritis, KneeAdipose TissueAnimalsGuinea PigsInflammation MediatorsKnee JointMaleObesitySynovial MembraneX-Ray MicrotomographyInflammation MediatorsBiomechanicsGaitHartley guinea pigInflammationInfrapatellar fat pad/synovium complexOsteoarthritisTrace elements

Identifiers

PMID36578046
PMCPMC9795160
OpenAlexW4313339930

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.