ArticleCell reports2022
Nuclear-cytoplasmic compartmentalization of cyclin B1-Cdk1 promotes robust timing of mitotic events.
Article in Cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- A Constellation of Fluorescent Biosensors to Illuminate the Galaxy of Protein Kinases.Chembiochem : a European journal of chemical biology · 2026Review
- Energy-precision trade-off in mitotic oscillators revealed by ATP modulation in artificial cells.bioRxiv : the preprint server for biology · 2026Article
- Article
- LncRNA H19-Encoded Micropeptide altH19 Promotes DNA Replication and Mitosis in Myeloma Cells by Enhancing the Phosphorylation of CDK2 at Threonine 160.Cell proliferation · 2026Article
- Mechanistic origins of temperature scaling in the early embryonic cell cycle.Nature communications · 2025Article
- E2F activity determines mitosis versus whole-genome duplication in G2-arrested cells.Nature communications · 2025Article
- Article
- Dengue virus modulates critical cell cycle regulatory proteins in human megakaryocyte cells.Scientific reports · 2025Article
- Capturing CDKs in action: Live-cell biosensors pioneer the new frontiers in cell cycle research.Cell structure and function · 2025Review
- Spatial heterogeneity accelerates phase-to-trigger wave transitions in frog egg extracts.Nature communications · 2024Article
- Seasonal modulation of the testis transcriptome reveals insights into hibernation and reproductive adaptation in Onychostoma macrolepis.Fish physiology and biochemistry · 2024Article
- Robust trigger wave speed in Xenopus cytoplasmic extracts.Nature communications · 2024Article
- Comprehensive Parameter Space Mapping of Cell Cycle Dynamics under Network Perturbations.ACS synthetic biology · 2024Article
- Mitotic waves in frog egg extracts: Transition from phase waves to trigger waves.bioRxiv : the preprint server for biology · 2024Article
- Collective effects of cell cleavage dynamics.Frontiers in cell and developmental biology · 2024Review
- Article
- Cyclin-dependent kinases: Masters of the eukaryotic universe.Wiley interdisciplinary reviews. RNA · 2023Review
- Characterization of factors that underlie transcriptional silencing in C. elegans oocytes.PLoS genetics · 2023Article
- Cell cycle oscillations driven by two interlinked bistable switches.Molecular biology of the cell · 2023Article
- CDK activity sensors: genetically encoded ratiometric biosensors for live analysis of the cell cycle.Biochemical Society transactions · 2022Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The cyclin-dependent kinase (Cdk1) oscillator is widely characterized in homogenized cytosolic extracts, leaving unclear the impact of nucleocytoplasmic compartmentalization. Here, by developing a Förster resonance energy transfer (FRET) biosensor, we track Cdk1 spatiotemporal dynamics in reconstituted cells with or without side by side and find compartmentalization significantly modulates clock properties previously found in bulk studies. Although nucleus-absent cells display highly tunable frequency, the nucleus-present cells maintain constant frequency against cyclin B1 variations. Despite high expression variability, cyclin degraded within the same duration, enabling a robust mitotic phase. Moreover, Cdk1 and cyclin B1 cycle rigorously out-of-phase, ensuring wide phase-plane orbits, essential for oscillation robustness. Although Cdk1 in homogeneous extracts is well known for delayed switch-like activation, we find active cyclin B1-Cdk1 accumulates in nuclei, without delay, until the nuclear envelope breakdown (NEB) when another abrupt activation triggers anaphase. Cdk1 biphasic activation and spatial compartmentalization may together coordinate the accurate ordering of different downstream events.
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