Evidence map›Paper›PMID 36575950›Full record

ArticleThe Journal of infectious diseases2023

Antigen Discovery for Next-Generation Pertussis Vaccines Using Immunoproteomics and Transposon-Directed Insertion Sequencing.

Kelsey A Gregg, Yihui Wang, Jason Warfel, Elizabeth Schoenfeld, Ewa Jankowska, John F Cipollo, Matthew Mayho, Christine Boinett, Deepika Prasad, Timothy J Brickman and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infectious diseases, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. CD4+ T-cell immunity toDiscover immunity · 2026
    Review
  6. Article
  7. Article
  8. Review
  9. Article
  10. Article
  11. Review
  12. Review
  13. Clinical microbiology reviews · 2023
    Review
  14. Article
  15. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Kelsey A GreggDivision of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.
Yihui WangDivision of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.
Jason WarfelDivision of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.
Elizabeth SchoenfeldDivision of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.
Ewa JankowskaDivision of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.
John F CipolloDivision of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.ORCID 0000-0002-9663-8177
Matthew MayhoWellcome Sanger Institute, Hinxton, United Kingdom.
Christine BoinettWellcome Sanger Institute, Hinxton, United Kingdom.
Deepika PrasadDivision of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.
Timothy J BrickmanDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.
Sandra K ArmstrongDepartment of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.
Julian ParkhillWellcome Sanger Institute, Hinxton, United Kingdom.
Ricardo Da Silva AntunesDivision of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, California, USA.
Alessandro SetteDivision of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, California, USA.
James F PapinDepartment of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.
Roman WolfDepartment of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.
Tod J MerkelDivision of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.

Funding

Large Scale T Cell Epitope Discovery: Genome-wide characterization of T cell epitopes from Bordetella pertussis in vaccination and natural infection75N93019C00066 · NIAID · LA JOLLA INSTITUTE FOR IMMUNOLOGY · PI SETTE, ALESSANDRO · 2019 to 2023
$4.5M
Identifying mechanisms and correlates of long-lasting vaccine-induced immunity to whooping coughU01AI141995 · NIAID · LA JOLLA INSTITUTE FOR IMMUNOLOGY · PI PETERS, BJOERN, SETTE, ALESSANDRO · 2019 to 2023
$4.1M
FDA HHSNIAID NIH HHS 75N93019C00066NIAID NIH HHS U01 AI141995NIH HHS 032521Wellcome Trust 098051
6 · The paper itself

Abstract

backgroundDespite high vaccination rates, the United States has experienced a resurgence in reported cases of pertussis after switching to the acellular pertussis vaccine, indicating a need for improved vaccines that enhance infection control.

methodsBordetella pertussis antigens recognized by convalescent-baboon serum and nasopharyngeal wash were identified by immunoproteomics and their subcellular localization predicted. Genes essential or important for persistence in the baboon airway were identified by transposon-directed insertion-site sequencing (TraDIS) analysis.

resultsIn total, 314 B. pertussis antigens were identified by convalescent baboon serum and 748 by nasopharyngeal wash. Thirteen antigens were identified as immunogenic in baboons, essential for persistence in the airway by TraDIS, and membrane-localized: BP0840 (OmpP), Pal, OmpA2, BP1485, BamA, Pcp, MlaA, YfgL, BP2197, BP1569, MlaD, ComL, and BP0183.

conclusionsThe B. pertussis antigens identified as immunogenic, essential for persistence in the airway, and membrane-localized warrant further investigation for inclusion in vaccines designed to reduce or prevent carriage of bacteria in the airway of vaccinated individuals.

Indexed as

Whooping CoughAnimalsAntibodies, BacterialBordetella pertussisHumansPapioPertussis VaccineAntibodies, BacterialPertussis VaccineantigenbaboonBordetella pertussisimmunizationimmunoproteomicsTraDISvaccinewhooping cough

Identifiers

PMID36575950
PMCPMC10169431

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.