ArticlePlant communications2023
MEP pathway products allosterically promote monomerization of deoxy-D-xylulose-5-phosphate synthase to feedback-regulate their supply.
Article in Plant communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Review of culture media and serum substitutes containing phytoecdysteroids for application in cultured meat technology.Food science of animal resources · 2026Review
- A Bacillus-based synthetic community: integrated growth promotion and control of fusarium root rot and black shank in tobacco.Pest management science · 2026Article
- Analysis of the DXS Pan-Gene Family Reveals Evolutionary Conservation and Regulatory Divergence Underpinning Terpenoid Metabolism in Lauraceae.Plants (Basel, Switzerland) · 2026Article
- Metabolic engineering of endogenous MEP pathway for enhanced lycopene production inSynthetic and systems biotechnology · 2026Article
- Metabolic Labeling and Visualization of Isoprenoids Using Alkyne-Modified DMAPP Analogs in Bacillus subtilis.Angewandte Chemie (International ed. in English) · 2026Article
- Enhancing Geranylgeranyl Pyrophosphate Supply in Engineered Cyanobacteria for Improved Production of Natural Pigments and Terpenoid Precursors.International journal of molecular sciences · 2026Article
- Beyond Tanacetum cinerariifolium: Advances in Pyrethrins Biosynthesis and Prospects for Engineered Cell Factories.Molecular biotechnology · 2026Review
- CHASE-independent cytokinin perception triggers 3',5'-cAMP signaling inJournal of bacteriology · 2026Article
- Harnessing evolution: leveraging bacterial isoprenoid pathway diversity toward improved bioengineering strategies.Journal of bacteriology · 2026Review
- Overview of Research on Essential Oils ofPharmaceuticals (Basel, Switzerland) · 2026Review
- Engineering Cyanobacteria for High-Yield Photosynthetic Isoprene Production With Long-Term Phenotypic Stability.Plant biotechnology journal · 2026Article
- Advanced applications of synthetic biology technology in biosynthesis of bioactive compounds from medicinal plants.Chinese herbal medicines · 2026Review
- Family alliances feeding the carotenoid pathway in tomato.Journal of experimental botany · 2025Review
- A single nucleotide substitution in the SlMCT gene contributes to great morphological alternations in tomato.Molecular horticulture · 2025Article
- Stable Isotopic Labeling of Dimethylallyl Pyrophosphate (DMAPP) Reveals Compartmentalization of Isoprenoid Biosynthesis during Sporulation inJournal of the American Chemical Society · 2025Article
- Article
- Metabolome and Transcriptome Combined Reveal the Main Floral Volatile Compounds and Key Regulatory Genes ofPlants (Basel, Switzerland) · 2024Article
- Rubisco supplies pyruvate for the 2-C-methyl-D-erythritol-4-phosphate pathway.Nature plants · 2024Article
- Arabidopsis VQ motif-containing proteins VQ1 and VQ10 interact with plastidial 1-deoxy-D-xylulose-5-phosphate synthase.Scientific reports · 2024Article
- Metabolic crosstalk between hydroxylated monoterpenes and salicylic acid in tomato defense response against bacteria.Plant physiology · 2024Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isoprenoids are a very large and diverse family of metabolites required by all living organisms. All isoprenoids derive from the double-bond isomers isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), which are produced by the methylerythritol 4-phosphate (MEP) pathway in bacteria and plant plastids. It has been reported that IPP and DMAPP feedback-regulate the activity of deoxyxylulose 5-phosphate synthase (DXS), a dimeric enzyme that catalyzes the main flux-controlling step of the MEP pathway. Here we provide experimental insights into the underlying mechanism. Isothermal titration calorimetry and dynamic light scattering approaches showed that IPP and DMAPP can allosterically bind to DXS in vitro, causing a size shift. In silico ligand binding site analysis and docking calculations identified a potential allosteric site in the contact region between the two monomers of the active DXS dimer. Modulation of IPP and DMAPP contents in vivo followed by immunoblot analyses confirmed that high IPP/DMAPP levels resulted in monomerization and eventual aggregation of the enzyme in bacterial and plant cells. Loss of the enzymatically active dimeric conformation allows a fast and reversible reduction of DXS activity in response to a sudden increase or decrease in IPP/DMAPP supply, whereas aggregation and subsequent removal of monomers that would otherwise be available for dimerization appears to be a more drastic response in the case of persistent IPP/DMAPP overabundance (e.g., by a blockage in their conversion to downstream isoprenoids). Our results represent an important step toward understanding the regulation of the MEP pathway and rational design of biotechnological endeavors aimed at increasing isoprenoid contents in microbial and plant systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.