Evidence map›Paper›PMID 36573588›Full record

ArticleJournal of animal science2023

Glycine regulates lipid peroxidation promoting porcine oocyte maturation and early embryonic development.

Lepeng Gao, Chang Zhang, Yingying Zheng, Deyi Wu, Xinyuan Chen, Hainan Lan, Xin Zheng, Hao Wu, Suo Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Lepeng GaoCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Chang ZhangCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Yingying ZhengCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Deyi WuCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Xinyuan ChenCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Hainan LanCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Xin ZhengCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Hao WuCOFCO Corporation, Beijing 100020, China.
Suo LiCollege of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.

Funding

Education Department of Jilin Province JJKH20220361KJNational Natural Science Foundation of China 31902162Project of Science and Technology
6 · The paper itself

Abstract

In vitro-cultured oocytes are separated from the follicular micro-environment in vivo and are more vulnerable than in vivo oocytes to changes in the external environment. This vulnerability disrupts the homeostasis of the intracellular environment, affecting oocyte meiotic completion, and subsequent embryonic developmental competence in vitro. Glycine, one of the main components of glutathione (GSH), plays an important role in the protection of porcine oocytes in vitro. However, the protective mechanism of glycine needs to be further clarified. Our results showed that glycine supplementation promoted cumulus cell expansion and oocyte maturation. Detection of oocyte development ability showed that glycine significantly increased the cleavage rate and blastocyst rate during in vitro fertilization (IVF). SMART-seq revealed that this effect was related to glycine-mediated regulation of cell membrane structure and function. Exogenous addition of glycine significantly increased the levels of the anti-oxidant GSH and the expression of anti-oxidant-related genes (glutathione peroxidase 4 [GPX4], catalase [CAT], superoxide dismutase 1 [SOD1], superoxide dismutase 2 [SOD2], and mitochondrial solute carrier family 25, member 39 [SLC25A39]), decreased the lipid peroxidation caused by reactive oxygen species (ROS) and reduced the level of malondialdehyde (MDA) by enhancing the functions of mitochondria, peroxisomes and lipid droplets (LDs) and the levels of lipid metabolism-related factors (peroxisome proliferator activated receptor coactivator 1 alpha [PGC-1α], peroxisome proliferator-activated receptor γ [PPARγ], sterol regulatory element binding factor 1 [SREBF1], autocrine motility factor receptor [AMFR], and ATP). These effects further reduced ferroptosis and maintained the normal structure and function of the cell membrane. Our results suggest that glycine plays an important role in oocyte maturation and later development by regulating ROS-induced lipid metabolism, thereby protecting against biomembrane damage.

Indexed as

AntioxidantsIn Vitro Oocyte Maturation TechniquesAnimalsBlastocystEmbryonic DevelopmentFemaleGlutathioneGlycineLipid PeroxidationOocytesPregnancyReactive Oxygen SpeciesSwineAntioxidantsGlutathioneGlycineReactive Oxygen Speciesferroptosisglycinelipid peroxidationoxidative stressporcine oocytes

Identifiers

PMID36573588
PMCPMC9904182

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.