ArticleEmerging infectious diseases2023
Molecular Tools for Early Detection of Invasive Malaria Vector Anopheles stephensi Mosquitoes.
Article in Emerging infectious diseases, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Field evaluation of SumiLarvMalaria journal · 2026Article
- Composition, blood meal sources, and entomological inoculation rates of Anopheles mosquitoes in an urban setting, central Ethiopia.Malaria journal · 2026Article
- Molecular xenomonitoring for identifying Plasmodium parasites and blood meal sources of mosquitoes in malaria endemic villages adjacent to the southern Rift Valley Lakes, South Ethiopia.Scientific reports · 2026Article
- Prioritizing urban water-storage containers for molecular surveillance of invasiveCurrent research in parasitology & vector-borne diseases · 2026Article
- Susceptibility of the invasive malaria vector Anopheles stephensi in Ethiopia to novel chemical insecticides and insect growth regulator.Scientific reports · 2025Article
- One-Step Multiplex Polymerase Chain Reaction Assay for the Detection of Major Disease-Transmitting Mosquito Vectors in India.The American journal of tropical medicine and hygiene · 2025Article
- Co-occurrence of native and invasive malaria vectors in anthropogenic habitats in Metehara, Ethiopia: Opportunities for urban malaria control.Current research in parasitology & vector-borne diseases · 2025Article
- Article
- The expansion of an invasive malaria vector: Anopheles stephensi emergence in Arba Minch town in the southern Rift Valley of Ethiopia.Parasitology research · 2024Article
- Loop-Mediated Isothermal Amplification Assay to Detect Invasive Malaria Vector Anopheles stephensi Mosquitoes.Emerging infectious diseases · 2024Article
- A decade of invasive Anopheles stephensi sequence-based identification: toward a global standard.Trends in parasitology · 2024Review
- Detection of Invasive Anopheles stephensi Mosquitoes through Molecular Surveillance, Ghana.Emerging infectious diseases · 2024Article
- Sibling species of the major malaria vector Anopheles gambiae display divergent preferences for aquatic breeding sites in southern Nigeria.Malaria journal · 2024Article
- Evidence for a role of Anopheles stephensi in the spread of drug- and diagnosis-resistant malaria in Africa.Nature medicine · 2023Article
- First detection ofbioRxiv : the preprint server for biology · 2023Article
- Detection of Anopheles stephensi Mosquitoes by Molecular Surveillance, Kenya.Emerging infectious diseases · 2023Article
- Citizen Science as an Approach for Responding to the Threat ofCitizen science : theory and practice · 2023Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reports of the expansion of the Asia malaria vector Anopheles stephensi mosquito into new geographic areas are increasing, which poses a threat to the elimination of urban malaria. Efficient surveillance of this vector in affected areas and early detection in new geographic areas is key to containing and controlling this species. To overcome the practical difficulties associated with the morphological identification of immature stages and adults of An. stephensi mosquitoes, we developed a species-specific PCR and a real-time PCR targeting a unique segment of the second internal transcribed spacer lacking homology to any other organism. Both PCRs can be used to identify An. stephensi mosquitoes individually or in pooled samples of mixed species, including when present in extremely low proportions (1:500). This study also reports a method for selective amplification and sequencing of partial ribosomal DNA from An. stephensi mosquitoes for their confirmation in pooled samples of mixed species.
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