ArticleParasites & vectors2022
Extensive testing of a multi-locus sequence typing scheme for Giardia duodenalis assemblage A confirms its good discriminatory power.
Article in Parasites & vectors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- High resolution multi-locus sequence typing scheme for Giardia duodenalis assemblage B outbreak and population analysis.PLoS neglected tropical diseases · 2026Article
- Comparison of multilocus genotyping and a commercial beta-giardin qPCR assay for detection of Giardia duodenalis zoonotic assemblages in cat and dog samples.Parasites & vectors · 2026Article
- Emergence of zoonoticFrontiers in microbiology · 2026Article
- Arginine metabolism has a pivotal function for the encystation of Giardia duodenalis.PLoS pathogens · 2026Article
- Comprehensive analysis of flavohemoprotein copy number variation in Giardia intestinalis: exploring links to metronidazole resistance.Parasites & vectors · 2024Article
- Autochthonous and imported giardiasis cases: An analysis of two decades of national surveillance data, Germany, 2002 to 2021.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Article
- Molecular epidemiology and multilocus genotyping ofParasite epidemiology and control · 2024Article
- Current Understanding ofCurrent tropical medicine reports · 2024Article
- Zoonotic Cryptosporidium and Giardia in marsupials-an update.Parasitology research · 2024Review
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12 authors.
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Abstract
backgroundThe flagellated parasite Giardia duodenalis is a major and global cause of diarrhoeal disease. Eight genetically very distinct groups, known as assemblages A to H, have been recognized in the G. duodenalis species complex, two of which (assemblages A and B) infect humans and other mammalian hosts. Informative typing schemes are essential to understand transmission pathways, characterize outbreaks and trace zoonotic transmission. In this study, we evaluated a published multi-locus sequence typing (MLST) scheme for G. duodenalis assemblage A, which is based on six polymorphic markers.
methodsWe genotyped 60 human-derived and 11 animal-derived G. duodenalis isolates collected in Europe and on other continents based on the published protocol. After retrieving previously published genotyping data and excluding isolates whose sequences showed allelic sequence heterozygosity, we analysed a dataset comprising 146 isolates.
resultsWe identified novel variants at five of the six markers and identified 78 distinct MLST types in the overall dataset. Phylogenetic interpretation of typing data confirmed that sub-assemblage AII only comprises human-derived isolates, whereas sub-assemblage AI comprises all animal-derived isolates and a few human-derived isolates, suggesting limited zoonotic transmission. Within sub-assemblage AII, isolates from two outbreaks, which occurred in Sweden and Italy, respectively, had unique and distinct MLST types. Population genetic analysis showed a lack of clustering by geographical origin of the isolates.
conclusionThe MLST scheme evaluated provides sufficient discriminatory power for epidemiological studies of G. duodenalis assemblage A.
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