Evidence map›Paper›PMID 36572886›Full record

ArticleJournal of orthopaedic surgery and research2022

Exosomes derived from miR-338-3p-modified adipose stem cells inhibited inflammation injury of chondrocytes via targeting RUNX2 in osteoarthritis.

ChunLiang Li, Wei Li, GengZang Pu, JingWen Wu, Feng Qin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of orthopaedic surgery and research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 33 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Review
  6. Review
  7. Review
  8. Review
  9. Article
  10. Article
  11. Review
  12. Review
  13. Review
  14. Review
  15. Article
  16. Article
  17. Review
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

ChunLiang LiDepartment of Orthopedic, Qinghai Provincial People's Hospital, Xining, 810006, Qinghai, China.
Wei LiDepartment of Orthopedic, Qinghai Provincial People's Hospital, Xining, 810006, Qinghai, China.
GengZang PuDepartment of Emergency Surgery, Qinghai Provincial People's Hospital, Xining, 810006, Qinghai, China.
JingWen WuDepartment of Emergency Surgery, Qinghai Provincial People's Hospital, Xining, 810006, Qinghai, China.
Feng QinDepartment of Endocrinology, Qinghai University Affiliated Hospital, Chengxi District, No. 6, Xichuan South Road, Xining, 810006, Qinghai, China. 2017900006@qhu.edu.cn.
Qinghai Provincial Peoples Hospital · CNQinghai University Affiliated Hospital · CN

Funding

Qinghai Science and Technology Department project 2019-ZJ-7067Young and middle-aged Scientific Research Project of Qinghai University Affiliated Hospital ASRF-2019-YB-18
6 · The paper itself

Abstract

backgroundOsteoarthritis (OA) is a chronic degenerative disease that is one of the main causes of disability in middle-aged and elderly people. Adipose stem cell (ASC)-derived exosomes (ASC-Exo) could repair cartilage damage and treat OA. MiRNA-338-3p expression was confirmed to play a role in inhibiting proinflammatory cytokines. Herein, we aimed to explore the mechanism by which exosomes derived from miR-338-3p overexpressing ASCs protects chondrocytes from interleukin (IL)-1β-induced chondrocyte change.

methodsExosomes were extracted from ASCs transfected with miR-338-3p or its antisense inhibitor. The ASC-Exos (miR-338-3p silencing/overexpression) were incubated with IL-1β-induced ATDC5 cells, followed by evaluation of the chondrocyte proliferation, degradation, and inflammation injury.

resultsIn vitro results revealed that ASC-Exos inhibited the expression of prostaglandin E2 (PGE2), IL-6, IL-1β, and TNF-α, as well as promoted the proliferation of ATDC5 cells. Moreover, ASC-Exos inhibited inflammation injury and degradation of ATDC5 cells by transferring miR-338-3p. Luciferase reporter assays showed that RUNX2 was a target gene of miR-338-3p. Additionally, RUNX2 overexpression in ATDC5 cells reversed the protective effect of miR-338-3p on chondrocytes. Taken together, this study demonstrated that exosomes secreted from miR-338-3p-modified ASCs were effective in the repair of IL-1β-induced chondrocyte change by inhibiting RUNX2 expression.

conclusionsOur result provided valuable data for understanding the mechanism of ASC-Exos in OA treatment.

Indexed as

ExosomesMicroRNAsOsteoarthritisAgedApoptosisChondrocytesCore Binding Factor Alpha 1 SubunitHumansInflammationInterleukin-1betaMiddle AgedStem CellsCore Binding Factor Alpha 1 SubunitInterleukin-1betaMicroRNAsMIRN338 microRNA, humanRUNX2 protein, humanExosomesMesenchymal stem cells from adipose tissuemiR-338-3pOsteoarthritis

Identifiers

PMID36572886
PMCPMC9791748
OpenAlexW4312218794

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.