Evidence map›Paper›PMID 36567331›Full record

ArticleBiotechnology for biofuels and bioproducts2022

Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts.

Chien-Yuan Lin, Gina M Geiselman, Di Liu, Harsha D Magurudeniya, Alberto Rodriguez, Yi-Chun Chen, Venkataramana Pidatala, Faride Unda, Bashar Amer, Edward E K Baidoo and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotechnology for biofuels and bioproducts, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.9field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 6 institutions in 2 countries.

Chien-Yuan Lin *DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.
Gina M Geiselman *DOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.
Di Liu *Department of Biomaterials and Biomanufacturing, Sandia National Laboratories, Livermore, CA, 94550, USA.
Harsha D MagurudeniyaDOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.
Alberto RodriguezDOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.
Yi-Chun ChenDOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.
Venkataramana PidatalaDOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.
Faride UndaDepartment of Wood Science, University of British Columbia, Vancouver, BC, Canada.
Bashar AmerDOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.
Edward E K BaidooDOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.
Shawn D MansfieldDepartment of Wood Science, University of British Columbia, Vancouver, BC, Canada.
Blake A SimmonsDOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.
Seema SinghDOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.
Henrik V SchellerDOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA.
John M GladdenDOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA. JMGladden@lbl.gov.
Aymerick EudesDOE Joint BioEnergy Institute, Emeryville, CA, 94608, USA. AGEudes@lbl.gov.
Lawrence Berkeley National Laboratory · USJoint BioEnergy Institute · USSandia National Laboratories California · USGreat Lakes Bioenergy Research Center · USUniversity of British Columbia · CAUniversity of California, Berkeley · US

Funding

the U. S. Department of Energy, Office of Science, Office of Biological and Environmental Research DE-AC02-05CH11231U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research DE-SC0018409
6 · The paper itself

Abstract

backgroundLignocellulosic resources are promising feedstocks for the manufacture of bio-based products and bioenergy. However, the inherent recalcitrance of biomass to conversion into simple sugars currently hinders the deployment of advanced bioproducts at large scale. Lignin is a primary contributor to biomass recalcitrance as it protects cell wall polysaccharides from degradation and can inhibit hydrolytic enzymes via non-productive adsorption. Several engineering strategies have been designed to reduce lignin or modify its monomeric composition. For example, expression of bacterial 3-dehydroshikimate dehydratase (QsuB) in poplar trees resulted in a reduction in lignin due to redirection of metabolic flux toward 3,4-dihydroxybenzoate at the expense of lignin. This reduction was accompanied with remarkable changes in the pools of aromatic compounds that accumulate in the biomass.

resultsThe impact of these modifications on downstream biomass deconstruction and conversion into advanced bioproducts was evaluated in the current study. Using ionic liquid pretreatment followed by enzymatic saccharification, biomass from engineered trees released more glucose and xylose compared to wild-type control trees under optimum conditions. Fermentation of the resulting hydrolysates using Rhodosporidium toruloides strains engineered to produce α-bisabolene, epi-isozizaene, and fatty alcohols showed no negative impact on cell growth and yielded higher titers of bioproducts (as much as + 58%) in the case of QsuB transgenics trees.

conclusionOur data show that low-recalcitrant poplar biomass obtained with the QsuB technology has the potential to improve the production of advanced bioproducts.

Indexed as

AromaticsFermentationIonic liquidRhodosporidium toruloidesSaccharificationWoody biomass

Identifiers

PMID36567331
PMCPMC9790118
OpenAlexW4312132030

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.