ArticleMicrobial cell factories2022
Combination of genetic engineering and random mutagenesis for improving production of raw-starch-degrading enzymes in Penicillium oxalicum.
Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Bioleaching for critical metal recovery from bauxite residue- unlocking waste valorization.Frontiers in bioengineering and biotechnology · 2025Review
- Regulation of genes encoding polysaccharide-degrading enzymes in Penicillium.Applied microbiology and biotechnology · 2024Review
- Enhancement of Digestive Enzyme Activity inMicroorganisms · 2024Article
- Mutual regulation of novel transcription factors RsrD and RsrE positively modulates the production of raw-starch-degrading enzyme inApplied and environmental microbiology · 2024Article
- A RsrC-RsrA-RsrB transcriptional circuit positively regulates polysaccharide-degrading enzyme biosynthesis and development in Penicillium oxalicum.Communications biology · 2024Article
- Selection and Genetic Analysis of High Polysaccharide-Producing Mutants inMicroorganisms · 2024Article
- Creating a novel genetic diversity ofHeliyon · 2024Article
- Strategies for the Development of Industrial Fungal Producing Strains.Journal of fungi (Basel, Switzerland) · 2023Review
- Kinase POGSK-3β modulates fungal plant polysaccharide-degrading enzyme production and development.Applied microbiology and biotechnology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundRaw starch-degrading enzyme (RSDE) is applied in biorefining of starch to produce biofuels efficiently and economically. At present, RSDE is obtained via secretion by filamentous fungi such as Penicillium oxalicum. However, high production cost is a barrier to large-scale industrial application. Genetic engineering is a potentially efficient approach for improving production of RSDE. In this study, we combined genetic engineering and random mutagenesis of P. oxalicum to enhance RSDE production.
resultsA total of 3619 mutated P. oxalicum colonies were isolated after six rounds of ethyl methanesulfonate and Co
conclusionsCombining genetic engineering and random mutagenesis efficiently enhanced production of RSDE by P. oxalicum. The RSDE-hyperproducing mutant GXUR001 was generated, and its crude RSDE could efficiently degrade raw starch. This strain has great potential for enzyme preparation and further genetic engineering.
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Registered trials
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