Evidence map›Paper›PMID 36565525›Full record

ArticleTalanta2023

Development of receptor binding domain-based double-antigen sandwich lateral flow immunoassay for the detection and evaluation of SARS-CoV-2 neutralizing antibody in clinical sera samples compared with the conventional virus neutralization test.

Ying Zhang, Yixiao Chen, Yong He, Yizhe Li, Xiaoli Zhang, Jiajie Liang, Jinyong He, Shaofang Lu, Zhixing Gao, Jianhua Xu and 1 more

Open access · greenAbstract read
In one paragraph

Article in Talanta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Ying ZhangDepartment of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, Jinan University, Guangzhou, 510632, PR China.
Yixiao ChenDepartment of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, Jinan University, Guangzhou, 510632, PR China.
Yong HeDepartment of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, Jinan University, Guangzhou, 510632, PR China.
Yizhe LiDepartment of Laboratory Science, Shunde Hospital of Guangzhou University of Chinese Medicine, Foshan, 528000, PR China.
Xiaoli ZhangDepartment of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, Jinan University, Guangzhou, 510632, PR China.
Jiajie LiangDepartment of Oncology, The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, 510632, China.
Jinyong HeDepartment of Laboratory Science, Shunde Hospital of Guangzhou University of Chinese Medicine, Foshan, 528000, PR China.
Shaofang LuDepartment of Laboratory Science, Shunde Hospital of Guangzhou University of Chinese Medicine, Foshan, 528000, PR China.
Zhixing GaoDepartment of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, Jinan University, Guangzhou, 510632, PR China.
Jianhua XuDepartment of Laboratory Science, Shunde Hospital of Guangzhou University of Chinese Medicine, Foshan, 528000, PR China. Electronic address: jhxu1976@gzucm.edu.cn.
Yong TangDepartment of Bioengineering, Guangdong Province Engineering Research Center of Antibody Drug and Immunoassay, Jinan University, Guangzhou, 510632, PR China. Electronic address: tyjaq7926@163.com.
Jinan University · CNGuangzhou University of Chinese Medicine · CNFirst Affiliated Hospital of Jinan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vaccination is an effective strategy to fight COVID-19. However, the effectiveness of the vaccine varies among different populations in varying immune effects. Neutralizing antibody (NAb) level is an important indicator to evaluate the protective effect of immune response after vaccination. Lateral flow immunoassay (LFIA) is a rapid, safe and sensitivity detection method, which has great potential in the detection of SARS-CoV-2 NAb. In this study, a fluorescent beads-based lateral flow immunoassay (FBs-LFIA) and a latex beads-based LFIA (LBs-LFIA) using double antigen sandwich (DAS) strategy were established to detect NAbs in the serum of vaccinated people. The limit of detection (LoD) of the FBs-LFIA was 1.13 ng mL

Indexed as

COVID-19SARS-CoV-2Antibodies, NeutralizingAntibodies, ViralAntigensHumansImmunoassayNeutralization TestsAntibodies, NeutralizingAntibodies, ViralAntigensDouble antigen sandwich (DAS)Lateral flow immunoassay (LFIA)Neutralizing antibodies (NAbs)Receptor-binding domain (RBD)SARS-CoV-2

Identifiers

PMID36565525
PMCPMC9767659
OpenAlexW4312030481

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.