Evidence map›Paper›PMID 36565355›Full record

ArticleBiomolecular NMR assignments2023

Justin A Grennell, Kendra D Jenkins, Kelvin B Luther, John Glushka, Robert S Haltiwanger, Megan A Macnaughtan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomolecular NMR assignments, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Justin A GrennellDepartment of Chemistry, Louisiana State University, Baton Range, LA, 70803, USA.
Kendra D JenkinsDepartment of Chemistry, Louisiana State University, Baton Range, LA, 70803, USA.
Kelvin B LutherDepartment of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, The University of Georgia, Athens, GA, 30602, USA.ORCID 0000-0001-9438-9675
John GlushkaDepartment of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, The University of Georgia, Athens, GA, 30602, USA.ORCID 0000-0002-6615-0496
Robert S HaltiwangerDepartment of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, The University of Georgia, Athens, GA, 30602, USA.ORCID 0000-0001-7439-9577
Megan A MacnaughtanDepartment of Chemistry, Louisiana State University, Baton Range, LA, 70803, USA. macnau@lsu.edu.ORCID 0000-0003-4626-0062

Funding

O-Glycosylation of Epidermal Growth Factor-like MotifsR01GM061126 · NIGMS · UNIVERSITY OF GEORGIA · PI HALTIWANGER, ROBERT S. · 2001 to 2021
$9.1M
NIGMS NIH HHS R01 GM061126NIH HHS GM061126
6 · The paper itself

Abstract

NOTCH1 is a transmembrane receptor in metazoans that is linked to a variety of disorders. The receptor contains an extracellular domain (ECD) with 36 tandem epidermal growth factor-like (EGF) repeats. The ECD is responsible for intercellular signaling via protein-ligand interactions with neighboring cells. Each EGF repeat consists of approximately 40 amino acids and 3 conserved disulfide bonds. The Abruptex region (EGF24-29) is critical for NOTCH1 signaling and is known for its missense mutations. Certain EGF repeats are modified with the addition of O-linked glycans and many have calcium binding sites, which give each EGF repeat a unique function. It has been shown that the loss of the O-fucose site of EGF27 alters NOTCH1 activity. To investigate the role of glycosylation in the NOTCH1 signaling pathway, nuclear magnetic resonance spectroscopy has been employed to study the structures of EGF27 and its glycoforms. Here, we report the backbone and sidechain

Indexed as

Epidermal Growth FactorReceptor, Notch1AnimalsBinding SitesGlycosylationMiceNuclear Magnetic Resonance, BiomolecularEpidermal Growth FactorReceptor, Notch1AbruptexEpidermal growth factor-like repeatExtracellular domainGlycosylationLunatic FringeNOTCH1POFUT1

Identifiers

PMID36565355
PMCPMC10626972

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.