ArticleMicrobial cell factories2022
Evolutionary and reverse engineering in Saccharomyces cerevisiae reveals a Pdr1p mutation-dependent mechanism for 2-phenylethanol tolerance.
Article in Microbial cell factories, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Yeast Stress Response to Synthetic Constructs.ACS synthetic biology · 2026Review
- EngineeringFrontiers in microbiology · 2026Review
- Metabolic reprogramming and computation-aided protein engineering for high-level de novo biosynthesis for 2-phenylethanol inSynthetic and systems biotechnology · 2025Article
- Reverse-Engineered Gas-Fermenting Acetogen Strains Recover Enhanced Phenotypes From Autotrophic Adaptive Laboratory Evolution.Microbial biotechnology · 2025Article
- Hybrid adaptation is hampered by Haldane's sieve.Nature communications · 2024Article
- Exploring the stress response mechanisms to 2-phenylethanol conferred by Pdr1p mutation in Saccharomyces cerevisiae.Biotechnology for biofuels and bioproducts · 2024Article
- Metabolic and tolerance engineering of Komagataella phaffii for 2-phenylethanol production through genome-wide scanning.Biotechnology for biofuels and bioproducts · 2024Article
- Identification of transporters involved in aromatic compounds tolerance through screening of transporter deletion libraries.Microbial biotechnology · 2024Article
- Contributions of Adaptive Laboratory Evolution towards the Enhancement of the Biotechnological Potential of Non-Conventional Yeast Species.Journal of fungi (Basel, Switzerland) · 2023Review
- Adaptive laboratory evolution to obtain furfural tolerantFrontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
background2-Phenylethanol (2-PE), a higher alcohol with a rose-like odor, inhibits growth of the producer strains. However, the limited knowledge regarding 2-PE tolerance mechanisms renders our current knowledge base insufficient to inform rational design.
resultsTo improve the growth phenotype of Saccharomyces cerevisiae under a high 2-PE concentration, adaptive laboratory evolution (ALE) was used to generate an evolved 19-2 strain. Under 2-PE stress, its OD
conclusionsIn the present work, we have demonstrated the use of ALE as a powerful tool to improve yeast tolerance to 2-PE. Based on the reverse engineering, transcriptional and physiological analysis, we concluded that Pdr1p mutation significantly enhanced the 2-PE tolerance of yeast by regulating the fatty acid proportion, intracellular ergosterol and ROS. It provides new insights on Pdr1p mediated 2-PE tolerance, which could help in the design of more robust yeasts for natural 2-PE synthesis.
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