Evidence map›Paper›PMID 36564750›Full record

ArticleMalaria journal2022

Transcriptional profiling of hepatocytes infected with the replicative form of the malaria parasite Plasmodium cynomolgi.

Gabriel Mitchell, Guglielmo Roma, Annemarie Voorberg-van der Wel, Martin Beibel, Anne-Marie Zeeman, Sven Schuierer, Laura Torres, Erika L Flannery, Clemens H M Kocken, Sebastian A Mikolajczak and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Malaria journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Transfection Models to InvestigatePathogens (Basel, Switzerland) · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 3 countries.

Gabriel MitchellOpen Innovation at Novartis Institute for Tropical Diseases, Novartis Institutes for BioMedical Research, Emeryville, CA, USA. gabriel.mitchell@novartis.com.
Guglielmo RomaChemical Biology & Therapeutics, Novartis Institutes for BioMedical Research, Basel, Switzerland.
Annemarie Voorberg-van der WelDepartment of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Martin BeibelChemical Biology & Therapeutics, Novartis Institutes for BioMedical Research, Basel, Switzerland.
Anne-Marie ZeemanDepartment of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Sven SchuiererChemical Biology & Therapeutics, Novartis Institutes for BioMedical Research, Basel, Switzerland.
Laura TorresOpen Innovation at Novartis Institute for Tropical Diseases, Novartis Institutes for BioMedical Research, Emeryville, CA, USA.
Erika L FlanneryNovartis Institute for Tropical Diseases, Novartis Institutes for BioMedical Research, Emeryville, CA, USA.
Clemens H M KockenDepartment of Parasitology, Biomedical Primate Research Centre, Rijswijk, The Netherlands.
Sebastian A MikolajczakNovartis Institute for Tropical Diseases, Novartis Institutes for BioMedical Research, Emeryville, CA, USA.
Thierry T DiaganaNovartis Institute for Tropical Diseases, Novartis Institutes for BioMedical Research, Emeryville, CA, USA.
Genomics Institute of the Novartis Research Foundation · USBiomedical Primate Research Centre · NLNovartis (Switzerland) · CH

Funding

Bill and Melinda Gates Foundation OPP1141292Wellcome TrustWellcome Trust WT078285
6 · The paper itself

Abstract

backgroundThe zoonotic simian parasite Plasmodium cynomolgi develops into replicating schizonts and dormant hypnozoites during the infection of hepatocytes and is used as a model organism to study relapsing malaria. The transcriptional profiling of P. cynomolgi liver stages was previously reported and revealed many important biological features of the parasite but left out the host response to malaria infection.

methodsPreviously published RNA sequencing data were used to quantify the expression of host genes in rhesus macaque hepatocytes infected with P. cynomolgi in comparison to either cells from uninfected samples or uninfected bystander cells.

resultsAlthough the dataset could not be used to resolve the transcriptional profile of hypnozoite-infected hepatocytes, it provided a snapshot of the host response to liver stage schizonts at 9-10 day post-infection and identified specific host pathways that are modulated during the exo-erythrocytic stage of P. cynomolgi.

conclusionsThis study constitutes a valuable resource characterizing the hepatocyte response to P. cynomolgi infection and provides a framework to build on future research that aims at understanding hepatocyte-parasite interactions during relapsing malaria infection.

Indexed as

MalariaParasitesPlasmodium cynomolgiAnimalsHepatocytesLiverMacaca mulattaHost responseHypnozoitesLiver cellsMonkeyRelapsing malariaRNA sequencingSchizonts

Identifiers

PMID36564750
PMCPMC9789591
OpenAlexW4312179121

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.