Evidence map›Paper›PMID 36564721›Full record

ArticleBMC plant biology2022

Influential factors and transcriptome analyses of immature diploid embryo anthocyanin accumulation in maize.

Chen Chen, Yuling Zhang, Xiuyi Fu, Chuanyong Chen, Shanshan Wu, Chunyuan Zhang, Huasheng Zhang, Yiyao Chang, Shaojiang Chen, Jiuran Zhao and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Chen ChenMaize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Yuling ZhangNational Maize Improvement Center of China, College of Agronomy and Biotechnology, Sanya Research Institute, China Agricultural University, Beijing, 100193, China.
Xiuyi FuMaize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Chuanyong ChenMaize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Shanshan WuMaize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Chunyuan ZhangMaize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Huasheng ZhangMaize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Yiyao ChangNational Maize Improvement Center of China, College of Agronomy and Biotechnology, Sanya Research Institute, China Agricultural University, Beijing, 100193, China.
Shaojiang ChenNational Maize Improvement Center of China, College of Agronomy and Biotechnology, Sanya Research Institute, China Agricultural University, Beijing, 100193, China.
Jiuran ZhaoMaize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. maizezhao@126.com.
Chenxu LiuNational Maize Improvement Center of China, College of Agronomy and Biotechnology, Sanya Research Institute, China Agricultural University, Beijing, 100193, China. liucx@cau.edu.cn.
Yuandong WangMaize Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China. wyuandong@126.com.
Beijing Academy of Agricultural and Forestry Sciences · CNChina Agricultural University · CN

Funding

BAAFS excellent scientist training program JKZX201902BAAFS science and technology innovation capability improvement project KJCX20200102Beijing Academy of Agricultural and Forestry Sciences Postdoctoral Research Foundation 2021-ZZ-020Beijing Postdoctoral Research Foundation 2022-ZZ-111China Agriculture Research System CARS-02Hainan Yazhou Bay Seed Laboratory project of JBGS mechanism B21HJ0501National Key Research and Development Program of China 2021YFD1201005
6 · The paper itself

Abstract

backgroundAnthocyanins are widely applied as a marker for haploid identification after haploid induction in maize. However, the factors affecting anthocyanin biosynthesis in immature embryos and the genes regulating this process remain unclear.

resultsIn this study, we analyzed the influence of genetic background of the male and female parents, embryo age and light exposure on anthocyanin accumulation in embryos. The results showed that light exposure was the most crucial factor enhancing the pigmentation of immature embryos. The identification accuracy of haploid embryos reached 96.4% after light exposure, but was only 11.0% following dark treatment. The total anthocyanin content was 7-fold higher in immature embryos cultured for 24 h under light conditions compared to embryos cultured in the dark. Transcriptome analysis revealed that the differentially expressed genes between immature embryos cultured for 24 h in dark and light chambers were significantly enriched in the pathways of flavonoid, flavone, flavonol and anthocyanin biosynthesis. Among the genes involved in anthocyanin biosynthesis, five up-regulated genes were identified: F3H, DFR, ANS, F3'H and the MYB transcription factor-encoding gene C1. The expression patterns of 14 selected genes were confirmed using quantitative reverse transcription-polymerase chain reaction.

conclusionLight is the most important factor facilitating anthocyanin accumulation in immature embryos. After 24 h of exposure to light, the expression levels of the structural genes F3H, DFR, ANS, F3'H and transcription factor gene C1 were significantly up-regulated. This study provides new insight into the factors and key genes regulating anthocyanin biosynthesis in immature embryos, and supports improved efficiency of immature haploid embryo selection during doubled haploid breeding of maize.

Indexed as

AnthocyaninsZea maysDiploidyGene Expression ProfilingGene Expression Regulation, PlantPlant BreedingPlant ProteinsTranscription FactorsTranscriptomeAnthocyaninsPlant ProteinsTranscription FactorsAnthocyanin biosynthesisHaploid identificationImmature haploid embryosMaize

Identifiers

PMID36564721
PMCPMC9789580
OpenAlexW4312203235

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.