Evidence map›Paper›PMID 36555213›Full record

ArticleInternational journal of molecular sciences2022

Targeted Methylation Profiling of Single Laser-Capture Microdissected Post-Mortem Brain Cells by Adapted Limiting Dilution Bisulfite Pyrosequencing (LDBSP).

Renzo J M Riemens, Gunter Kenis, Jennifer Nolz, Sonia C Susano Chaves, Diane Duroux, Ehsan Pishva, Diego Mastroeni, Kristel Van Steen, Thomas Haaf, Daniël L A van den Hove

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. State-of-the-Art Molecular Genetics and Genomics in Germany.International journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 4 countries.

Renzo J M RiemensDepartment of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience (MHeNs), Maastricht University, 6229 ER Maastricht, The Netherlands.ORCID 0000-0003-0280-6174
Gunter KenisDepartment of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience (MHeNs), Maastricht University, 6229 ER Maastricht, The Netherlands.
Jennifer NolzBiodesign Institute, Neurodegenerative Disease Research Center, Arizona State University, Tempe, AZ 85281, USA.
Sonia C Susano ChavesDepartment of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience (MHeNs), Maastricht University, 6229 ER Maastricht, The Netherlands.ORCID 0000-0001-6805-2497
Diane DurouxBIO3 Laboratory for Systems Genetics, GIGA-R Medical Genomics, University of Liège, 4000 Liège, Belgium.ORCID 0000-0002-8564-3157
Ehsan PishvaDepartment of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience (MHeNs), Maastricht University, 6229 ER Maastricht, The Netherlands.
Diego MastroeniBiodesign Institute, Neurodegenerative Disease Research Center, Arizona State University, Tempe, AZ 85281, USA.
Kristel Van SteenBIO3 Laboratory for Systems Genetics, GIGA-R Medical Genomics, University of Liège, 4000 Liège, Belgium.
Thomas HaafInstitute of Human Genetics, Julius Maximilians University, 97074 Wuerzburg, Germany.ORCID 0000-0002-0737-0763
Daniël L A van den HoveDepartment of Psychiatry and Neuropsychology, School for Mental Health and Neuroscience (MHeNs), Maastricht University, 6229 ER Maastricht, The Netherlands.ORCID 0000-0003-4047-3198
Maastricht University · NLArizona State University · USUniversity of Liège · BEUniversity of Würzburg · DE

Funding

European Union's Horizon 2020 / Marie Skłodowska-Curie 813533 860895, and 643417Joint Programme-Neurodegenerative Disease Research (JPND) / European Union's Horizon 2020 643417
6 · The paper itself

Abstract

A reoccurring issue in neuroepigenomic studies, especially in the context of neurodegenerative disease, is the use of (heterogeneous) bulk tissue, which generates noise during epigenetic profiling. A workable solution to this issue is to quantify epigenetic patterns in individually isolated neuronal cells using laser capture microdissection (LCM). For this purpose, we established a novel approach for targeted DNA methylation profiling of individual genes that relies on a combination of LCM and limiting dilution bisulfite pyrosequencing (LDBSP). Using this approach, we determined cytosine-phosphate-guanine (CpG) methylation rates of single alleles derived from 50 neurons that were isolated from unfixed post-mortem brain tissue. In the present manuscript, we describe the general workflow and, as a showcase, demonstrate how targeted methylation analysis of various genes, in this case,

Indexed as

Neurodegenerative DiseasesApoptosis Regulatory ProteinsBrainDNA MethylationHigh-Throughput Nucleotide SequencingHumansLasersMolecular ChaperonesSequence Analysis, DNASulfitesApoptosis Regulatory ProteinsDNAJB13 protein, humanhydrogen sulfiteMolecular ChaperonesSulfitesDNA methylationepigeneticslaser-capture microdissectionlimiting dilution bisulfite pyrosequencingsingle-cell

Identifiers

PMID36555213
PMCPMC9779089
OpenAlexW4311957211

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.